TY - JOUR
T1 - Irreversible electroporation attenuates neointimal formation after angioplasty
AU - Maor, Elad
AU - Ivorra, Antoni
AU - Leor, Jonathan
AU - Rubinsky, Boris
N1 - Funding Information:
Manuscript received January 30, 2008; revised March 8, 2008. The work of B. Rubinsky was supported by the Israel Science Foundation under Grant 403/06. Asterisk indicates corresponding author. ∗E. Maor is with the Biophysics Graduate Group, University of California, Berkeley, CA 94720 USA (e-mail: [email protected]). A. Ivorra is with the University of California, Berkeley, CA 94720 USA. J. Leor is with the Neufeld Cardiac Research Institute, Sheba Medical Center, Tel Aviv University, Tel Hashomer 52621, Israel. B. Rubinsky is with the School of Computer Science and Engineering Hebrew University of Jerusalem, 91904 Jerusalem, Israel. He is also with the Department of Mechanical Engineering, Department of Bioengineering, University of California, Berkeley, CA 94720 USA. Color versions of one or more of the figures in this paper are available online at http://ieeexplore.ieee.org. Digital Object Identifier 10.1109/TBME.2008.923909
PY - 2008/9
Y1 - 2008/9
N2 - Restenosis following coronary angioplasty represents a major clinical problem. Irreversible electroporation (IRE) is a nonthermal, nonpharmacological cell ablation method. IRE utilizes a sequence of electrical pulses that produce permanent damage to tissue within a few seconds. Methods and results: The left carotid arteries of eight rats underwent in vivo intimal damage using two Fogarty angioplasty catheters. The procedure was immediately followed by IRE ablation in four rats, while the remaining four were used as the control group. The IRE ablation was performed using a sequence of ten dc pulses of 3800 V/cm, 100 μs each, at a frequency of ten pulses per second, applied across the blood vessel between two parallel electrodes. The electrical conductance of the treated tissue was measured during the electroporation to provide real-time feedback of the process. Left carotid arteries were excised and fixated after a 28-day follow-up period. Neointimal formation was evaluated histologically. The use of IRE was successful in three out of four animals in a way that is consistent with the measurements of blood vessel electrical properties. The integrity of the endothelial layer was recovered in the IRE-treated animals, compared with control. Successful IRE reduced neointima to media ratio (0.57 ± versus 1.88 ± 1.0, P = 0.02). Conclusions: We report for the first time the in vivo results of attenuation of neointimal formation using IRE. Our study shows that IRE might be able to attenuate neointimal formation after angioplasty damage in a rodent model of restenosis. This approach may open new venues in the treatment of coronary artery restenosis after balloon angioplasty.
AB - Restenosis following coronary angioplasty represents a major clinical problem. Irreversible electroporation (IRE) is a nonthermal, nonpharmacological cell ablation method. IRE utilizes a sequence of electrical pulses that produce permanent damage to tissue within a few seconds. Methods and results: The left carotid arteries of eight rats underwent in vivo intimal damage using two Fogarty angioplasty catheters. The procedure was immediately followed by IRE ablation in four rats, while the remaining four were used as the control group. The IRE ablation was performed using a sequence of ten dc pulses of 3800 V/cm, 100 μs each, at a frequency of ten pulses per second, applied across the blood vessel between two parallel electrodes. The electrical conductance of the treated tissue was measured during the electroporation to provide real-time feedback of the process. Left carotid arteries were excised and fixated after a 28-day follow-up period. Neointimal formation was evaluated histologically. The use of IRE was successful in three out of four animals in a way that is consistent with the measurements of blood vessel electrical properties. The integrity of the endothelial layer was recovered in the IRE-treated animals, compared with control. Successful IRE reduced neointima to media ratio (0.57 ± versus 1.88 ± 1.0, P = 0.02). Conclusions: We report for the first time the in vivo results of attenuation of neointimal formation using IRE. Our study shows that IRE might be able to attenuate neointimal formation after angioplasty damage in a rodent model of restenosis. This approach may open new venues in the treatment of coronary artery restenosis after balloon angioplasty.
KW - Angioplasty
KW - Bioelectric phenomena
KW - Biomedical engineering
KW - Biophysics
KW - Blood vessels
KW - Cardiology
KW - Electric field effects
KW - Electroporation
KW - Restenosis
UR - http://www.scopus.com/inward/record.url?scp=50049127073&partnerID=8YFLogxK
U2 - 10.1109/TBME.2008.923909
DO - 10.1109/TBME.2008.923909
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AN - SCOPUS:50049127073
SN - 0018-9294
VL - 55
SP - 2268
EP - 2274
JO - IEEE Transactions on Biomedical Engineering
JF - IEEE Transactions on Biomedical Engineering
IS - 9
M1 - 20
ER -