Irradiation enhances the metastatic potential of prostatic small cell carcinoma xenografts

Lilach Agemy, Alon Harmelin, Tova Waks, Ilan Leibovitch, Tatyana Rabin, M. Raphael Pfeffer, Zelig Eshhar

Research output: Contribution to journalArticlepeer-review

Abstract

BACKGROUND. Small cell carcinoma of the prostate (SCCP) is a rare subset of prostate cancer (0.5-2% of all prostatic carcinomas), predominantly composed of neuroendocrine (NE) cells, with a very poor prognosis. Irradiation is one of the mainstay options for SCCP local treatment, yet, little is known about the clinical response of these aggressive tumors to radiotherapy. METHODS. Using SCID mice, the response to fractionated ionizing radiation (IR) of two unique human NE xenografts of SCCP (WISH-PC2 and WM-4A) was investigated. RESULTS. Fractionated irradiation of WISH-PC2 xenografts using total doses of >24 Gy induced a delay in tumor growth, while total doses of >36 Gy led to local tumor eradication. However, most of the irradiated mice suffered from disseminated metastases. Similarly, in the WM-4A xenograft, a total dose of 20 Gy led to tumor growth delay and some of the mice also developed metastases. Non-irradiated local xenografts failed to disseminate, even following surgical excision of the main tumor mass; however, tumor cells administered intravenously did form metastases. Metastases of both xenografts were located in the adrenal/kidney and interscapular regions, areas rich in brown adipose tissue. A correlation was found between the appearance of irradiation-induced metastases and activation of the gelatinase activity of matrix metalloproteinase-9. CONCLUSIONS. Clinically, this study raises the possibility that radiation to SCCP may promote metastatic disease. For patients in whom prostate biopsy shows a predominance of small cell cancer, it may be necessary to deliver systemic therapy together with the radiotherapy in order to prevent the development of metastases.

Original languageEnglish
Pages (from-to)530-539
Number of pages10
JournalProstate
Volume68
Issue number5
DOIs
StatePublished - 1 Apr 2008
Externally publishedYes

Keywords

  • Ionizing radiation
  • Matrix metalloproteinase
  • Metastasis
  • Small cell carcinoma of the prostate

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