TY - JOUR
T1 - IR-biosensor
AU - Bindig, U.
AU - Meinke, M.
AU - Gersonde, I.
AU - Spector, O.
AU - Vasserman, I.
AU - Katzir, A.
AU - Müller, G.
N1 - Funding Information:
This work was supported by the German Ministry for Education, Science, Research and Technology, Project No. 13N7064.
PY - 2001/4/15
Y1 - 2001/4/15
N2 - Cylindrical optical fibers, made of various materials, have been used for decades for chemical and biological sensing. Optical fibers transparent in the mid-infrared (IR) enable absorption measurements in remote locations. New techniques will enable the use of IR fibers for measurements in ATR-mode. If the fiber is in contact with a sample that has characteristic absorption lines, the total transmission of the fiber and sample of these lines will decrease. In this way the absorption of a sample can be determined using a non-destructive method. This work proves that flattened silver halide fibers could be useful as sensing elements in analytical systems. First measurements using flattened IR-fibers have been carried out on biological targets. The experimental set-up used was made up of an FTIR-spectrometer, fiber optic cables and an external detector. A segment of 1.9 cm at the center of the fiber was flattened to approximately 100-150 μm. Transmission spectra of flattened fibers have been compared to those of the cylindrical fibers. IR-spectra were recorded for biochemical compounds such as glucose, bovine serum albumin (BSA), gelatin and bio-fluids, and native tissue was compared to coagulated tissue. Spectral differences were obtained indicating a possible use for the study of thermal damage in tissues.
AB - Cylindrical optical fibers, made of various materials, have been used for decades for chemical and biological sensing. Optical fibers transparent in the mid-infrared (IR) enable absorption measurements in remote locations. New techniques will enable the use of IR fibers for measurements in ATR-mode. If the fiber is in contact with a sample that has characteristic absorption lines, the total transmission of the fiber and sample of these lines will decrease. In this way the absorption of a sample can be determined using a non-destructive method. This work proves that flattened silver halide fibers could be useful as sensing elements in analytical systems. First measurements using flattened IR-fibers have been carried out on biological targets. The experimental set-up used was made up of an FTIR-spectrometer, fiber optic cables and an external detector. A segment of 1.9 cm at the center of the fiber was flattened to approximately 100-150 μm. Transmission spectra of flattened fibers have been compared to those of the cylindrical fibers. IR-spectra were recorded for biochemical compounds such as glucose, bovine serum albumin (BSA), gelatin and bio-fluids, and native tissue was compared to coagulated tissue. Spectral differences were obtained indicating a possible use for the study of thermal damage in tissues.
KW - Coagulation
KW - Fiber evanescent wave spectroscopy (FEWS)
KW - Flattened IR-fiber
KW - Tissue
UR - http://www.scopus.com/inward/record.url?scp=0035871885&partnerID=8YFLogxK
U2 - 10.1016/S0925-4005(00)00709-7
DO - 10.1016/S0925-4005(00)00709-7
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AN - SCOPUS:0035871885
SN - 0925-4005
VL - 74
SP - 37
EP - 46
JO - Sensors and Actuators B: Chemical
JF - Sensors and Actuators B: Chemical
IS - 1-3
Y2 - 16 April 2000 through 19 April 2000
ER -