Involvement of singlet oxygen in photoactivation of aflatoxins B1 and B2 to DNA-binding forms in vitro

Avishay Abraham Stark; Yana Gal; Gad Shaulsky

doi:10.1093/carcin/11.4.529

Involvement of singlet oxygen in photoactivation of aflatoxins B₁ and B₂ to DNA-binding forms in vitro

Avishay Abraham Stark^*
, Yana Gal
, Gad Shaulsky

^*Corresponding author for this work

Biochemistry Molecular Biology

Tel Aviv University

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

UVA-activated aflatoxin B₁ (AFB₁) and AFB₂ bound covalently to DNA. The rate of binding increased in D₂O versus H₂O and decreased by diazabicyclo-odane, azide, aniline, 2-naphthylamine and by efficient strip ing of oxygen. P Similarly to DNA-binding, singlet oxygen (¹O₂ formation increased in D₂O and was inhibited by singlet scavengers, as judged by the formation of malonaldehyde from linolenic acid. The above argue for the involvement of ¹O₂ in aflatoxin photoactivation to DNA-binding species. A model for the mechanism is proposed.

Original language	English
Pages (from-to)	529-534
Number of pages	6
Journal	Carcinogenesis
Volume	11
Issue number	4
DOIs	https://doi.org/10.1093/carcin/11.4.529
State	Published - Apr 1990

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title = "Involvement of singlet oxygen in photoactivation of aflatoxins B1 and B2 to DNA-binding forms in vitro",

abstract = "UVA-activated aflatoxin B1 (AFB1) and AFB2 bound covalently to DNA. The rate of binding increased in D2O versus H2O and decreased by diazabicyclo-odane, azide, aniline, 2-naphthylamine and by efficient strip ing of oxygen. P Similarly to DNA-binding, singlet oxygen (1O2 formation increased in D2O and was inhibited by singlet scavengers, as judged by the formation of malonaldehyde from linolenic acid. The above argue for the involvement of 1O2 in aflatoxin photoactivation to DNA-binding species. A model for the mechanism is proposed.",

author = "Stark, \{Avishay Abraham\} and Yana Gal and Gad Shaulsky",

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AU - Stark, Avishay Abraham

AU - Gal, Yana

AU - Shaulsky, Gad

PY - 1990/4

Y1 - 1990/4

N2 - UVA-activated aflatoxin B1 (AFB1) and AFB2 bound covalently to DNA. The rate of binding increased in D2O versus H2O and decreased by diazabicyclo-odane, azide, aniline, 2-naphthylamine and by efficient strip ing of oxygen. P Similarly to DNA-binding, singlet oxygen (1O2 formation increased in D2O and was inhibited by singlet scavengers, as judged by the formation of malonaldehyde from linolenic acid. The above argue for the involvement of 1O2 in aflatoxin photoactivation to DNA-binding species. A model for the mechanism is proposed.

AB - UVA-activated aflatoxin B1 (AFB1) and AFB2 bound covalently to DNA. The rate of binding increased in D2O versus H2O and decreased by diazabicyclo-odane, azide, aniline, 2-naphthylamine and by efficient strip ing of oxygen. P Similarly to DNA-binding, singlet oxygen (1O2 formation increased in D2O and was inhibited by singlet scavengers, as judged by the formation of malonaldehyde from linolenic acid. The above argue for the involvement of 1O2 in aflatoxin photoactivation to DNA-binding species. A model for the mechanism is proposed.

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JO - Carcinogenesis

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ER -

Involvement of singlet oxygen in photoactivation of aflatoxins B₁ and B₂ to DNA-binding forms in vitro

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