TY - JOUR
T1 - Involvement of singlet oxygen in photoactivation of aflatoxins B1 and B2 to DNA-binding forms in vitro
AU - Stark, Avishay Abraham
AU - Gal, Yana
AU - Shaulsky, Gad
PY - 1990/4
Y1 - 1990/4
N2 - UVA-activated aflatoxin B1 (AFB1) and AFB2 bound covalently to DNA. The rate of binding increased in D2O versus H2O and decreased by diazabicyclo-odane, azide, aniline, 2-naphthylamine and by efficient strip ing of oxygen. P Similarly to DNA-binding, singlet oxygen (1O2 formation increased in D2O and was inhibited by singlet scavengers, as judged by the formation of malonaldehyde from linolenic acid. The above argue for the involvement of 1O2 in aflatoxin photoactivation to DNA-binding species. A model for the mechanism is proposed.
AB - UVA-activated aflatoxin B1 (AFB1) and AFB2 bound covalently to DNA. The rate of binding increased in D2O versus H2O and decreased by diazabicyclo-odane, azide, aniline, 2-naphthylamine and by efficient strip ing of oxygen. P Similarly to DNA-binding, singlet oxygen (1O2 formation increased in D2O and was inhibited by singlet scavengers, as judged by the formation of malonaldehyde from linolenic acid. The above argue for the involvement of 1O2 in aflatoxin photoactivation to DNA-binding species. A model for the mechanism is proposed.
UR - http://www.scopus.com/inward/record.url?scp=0025230784&partnerID=8YFLogxK
U2 - 10.1093/carcin/11.4.529
DO - 10.1093/carcin/11.4.529
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AN - SCOPUS:0025230784
SN - 0143-3334
VL - 11
SP - 529
EP - 534
JO - Carcinogenesis
JF - Carcinogenesis
IS - 4
ER -
