Involvement of singlet oxygen in photoactivation of aflatoxins B1 and B2 to DNA-binding forms in vitro

Avishay Abraham Stark*, Yana Gal, Gad Shaulsky

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

UVA-activated aflatoxin B1 (AFB1) and AFB2 bound covalently to DNA. The rate of binding increased in D2O versus H2O and decreased by diazabicyclo-odane, azide, aniline, 2-naphthylamine and by efficient strip ing of oxygen. P Similarly to DNA-binding, singlet oxygen (1O2 formation increased in D2O and was inhibited by singlet scavengers, as judged by the formation of malonaldehyde from linolenic acid. The above argue for the involvement of 1O2 in aflatoxin photoactivation to DNA-binding species. A model for the mechanism is proposed.

Original languageEnglish
Pages (from-to)529-534
Number of pages6
JournalCarcinogenesis
Volume11
Issue number4
DOIs
StatePublished - Apr 1990

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