TY - JOUR
T1 - Involvement of pituitary adenylate cyclase-activating polypeptide II vasoactive intestinal peptide 2 receptor in mouse neocortical astrocytogenesis
AU - Zupan, Véronique
AU - Hill, Joanna M.
AU - Brenneman, Douglas E.
AU - Gozes, Illana
AU - Fridkin, Mati
AU - Robberecht, Patrick
AU - Evrard, Philippe
AU - Gressens, Pierre
PY - 1998/5
Y1 - 1998/5
N2 - At the end of neuronal migration, the neopallial germinative zone produces glial cells destined to colonize the upper layers of neocortex. High densities of binding sites for vasoactive intestinal peptide (VIP) have been found in the rodent germinative zone just after completion of neuronal migration, suggesting a possible role of VIP in neocortical astrocytogenesis. In the present study, administration of a VIP antagonist at embryonic days 17 and 18 to pregnant mice was followed by a dramatic depletion of astrocytes in the upper cortical layer of the offspring. The depletion of astrocytes was dose-dependent, with a 42% reduction in the density of astrocytes observed with 50 μg of antagonist. The antagonist effect was reversed by cotreatment with VIP or pituitary adenylate cyclase-activating polypeptide (PACAP), suggesting the involvement of a receptor common to these two neuropeptides. VIP antagonist-induced inhibition of astrocytogenesis was also blocked by Ro 25-1553, a long-acting cyclic VIP analogue selective for the PACAP II VIP2 receptor subclass. Our results demonstrate that VIP and/or PACAP play a crucial physiological role in neocortical astrocytogenesis, possibly through interaction with PACAP II VIP2 receptors.
AB - At the end of neuronal migration, the neopallial germinative zone produces glial cells destined to colonize the upper layers of neocortex. High densities of binding sites for vasoactive intestinal peptide (VIP) have been found in the rodent germinative zone just after completion of neuronal migration, suggesting a possible role of VIP in neocortical astrocytogenesis. In the present study, administration of a VIP antagonist at embryonic days 17 and 18 to pregnant mice was followed by a dramatic depletion of astrocytes in the upper cortical layer of the offspring. The depletion of astrocytes was dose-dependent, with a 42% reduction in the density of astrocytes observed with 50 μg of antagonist. The antagonist effect was reversed by cotreatment with VIP or pituitary adenylate cyclase-activating polypeptide (PACAP), suggesting the involvement of a receptor common to these two neuropeptides. VIP antagonist-induced inhibition of astrocytogenesis was also blocked by Ro 25-1553, a long-acting cyclic VIP analogue selective for the PACAP II VIP2 receptor subclass. Our results demonstrate that VIP and/or PACAP play a crucial physiological role in neocortical astrocytogenesis, possibly through interaction with PACAP II VIP2 receptors.
KW - 5-Bromo-2'-deoxyuridine
KW - Activity-dependent neurotrophic factor
KW - Cyclic AMP
KW - Glial fibrillary acidic protein
KW - Growth factor
KW - Ro 25-1553
UR - http://www.scopus.com/inward/record.url?scp=0031969964&partnerID=8YFLogxK
U2 - 10.1046/j.1471-4159.1998.70052165.x
DO - 10.1046/j.1471-4159.1998.70052165.x
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AN - SCOPUS:0031969964
SN - 0022-3042
VL - 70
SP - 2165
EP - 2173
JO - Journal of Neurochemistry
JF - Journal of Neurochemistry
IS - 5
ER -