Involvement of CD24 in angiogenesis in a mouse model of oxygen-induced retinopathy

Hadas Newman, Shiran Shapira, Oriel Spierer*, Sarah Kraus, Mordechai Rosner, Sarah Pri-Chen, Anat Loewenstein, Nadir Arber, Adiel Barak

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Purpose: To investigate a possible involvement of CD24 in vascular remodeling and angiogenesis in retinopathy of prematurity (ROP) in a mouse model of oxygen-induced retinopathy. Materials and methods: 17 CD24 knockout (KO) and 12 wild-type (WT) C57BL/6 mice were used. Group 1 mice were exposed to oxygen concentrations of 75±2% from postnatal day (P) 7 to P12. Group 2 mice were raised in room air. At P17, all mice underwent fluorescein-conjugated- dextran perfusion and were sacrificed. The flat-mounted retinas were scored manually and digitally by a new computerized algorithm, according to blood vessel obliteration, tortuosity, vascular tufts and neovascularization formation. Results: Fifty four retinal whole mounts were available for analysis and scoring. Group 1 retinas had significantly higher values of vaso-obliteration, tufts, neovascularization, vessel tortuosity and higher mean retinopathy scores than Group 2 retinas (KO mice: 9.0±0.27 vs. 0.74±0.2, respectively, P<0.0001; WT mice: 7.58±0.40 vs. 1.17±0.27, respectively, P<0.0001). Manual scoring in Group 1 revealed higher values of neovascularization, tortuosity and mean retinopathy scores in KO mice vs. WT mice (9.0±0.27 vs. 7.58±0.40, respectively, P0.009). Digital scoring revealed a higher neovascularization score in KO mice as well (13.72±0.82% vs. 8.06±0.27%, P<0.0001). All mice had similar vaso-obliteration areas. There were no significant differences between KO and WT mice in Group 2. Conclusions: Absence of CD24 may have a deleterious effect on angiogenesis occurring in the second stage of ROP development, though its role in vessel obliteration during the first stage of ROP is probably limited.

Original languageEnglish
Pages (from-to)532-539
Number of pages8
JournalCurrent Eye Research
Volume37
Issue number6
DOIs
StatePublished - Jun 2012

Keywords

  • Angiogenesis
  • CD24
  • Oxygen-induced retinopathy
  • Retinopathy of prematurity

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