TY - JOUR
T1 - Involvement of CD24 in angiogenesis in a mouse model of oxygen-induced retinopathy
AU - Newman, Hadas
AU - Shapira, Shiran
AU - Spierer, Oriel
AU - Kraus, Sarah
AU - Rosner, Mordechai
AU - Pri-Chen, Sarah
AU - Loewenstein, Anat
AU - Arber, Nadir
AU - Barak, Adiel
N1 - Funding Information:
Andreas Stahl, MD, the Lois Smith Lab, Harvard Medical School, Children’s Hospital Boston. For incorporation of a new algorithm for quantification of green retinas into the SWIFT_NV method. This study was supported by a grant from the Claire and Amedee Maratier Institute for the Study of Blindness and Visual Disorders, Tel-Aviv University.
PY - 2012/6
Y1 - 2012/6
N2 - Purpose: To investigate a possible involvement of CD24 in vascular remodeling and angiogenesis in retinopathy of prematurity (ROP) in a mouse model of oxygen-induced retinopathy. Materials and methods: 17 CD24 knockout (KO) and 12 wild-type (WT) C57BL/6 mice were used. Group 1 mice were exposed to oxygen concentrations of 75±2% from postnatal day (P) 7 to P12. Group 2 mice were raised in room air. At P17, all mice underwent fluorescein-conjugated- dextran perfusion and were sacrificed. The flat-mounted retinas were scored manually and digitally by a new computerized algorithm, according to blood vessel obliteration, tortuosity, vascular tufts and neovascularization formation. Results: Fifty four retinal whole mounts were available for analysis and scoring. Group 1 retinas had significantly higher values of vaso-obliteration, tufts, neovascularization, vessel tortuosity and higher mean retinopathy scores than Group 2 retinas (KO mice: 9.0±0.27 vs. 0.74±0.2, respectively, P<0.0001; WT mice: 7.58±0.40 vs. 1.17±0.27, respectively, P<0.0001). Manual scoring in Group 1 revealed higher values of neovascularization, tortuosity and mean retinopathy scores in KO mice vs. WT mice (9.0±0.27 vs. 7.58±0.40, respectively, P0.009). Digital scoring revealed a higher neovascularization score in KO mice as well (13.72±0.82% vs. 8.06±0.27%, P<0.0001). All mice had similar vaso-obliteration areas. There were no significant differences between KO and WT mice in Group 2. Conclusions: Absence of CD24 may have a deleterious effect on angiogenesis occurring in the second stage of ROP development, though its role in vessel obliteration during the first stage of ROP is probably limited.
AB - Purpose: To investigate a possible involvement of CD24 in vascular remodeling and angiogenesis in retinopathy of prematurity (ROP) in a mouse model of oxygen-induced retinopathy. Materials and methods: 17 CD24 knockout (KO) and 12 wild-type (WT) C57BL/6 mice were used. Group 1 mice were exposed to oxygen concentrations of 75±2% from postnatal day (P) 7 to P12. Group 2 mice were raised in room air. At P17, all mice underwent fluorescein-conjugated- dextran perfusion and were sacrificed. The flat-mounted retinas were scored manually and digitally by a new computerized algorithm, according to blood vessel obliteration, tortuosity, vascular tufts and neovascularization formation. Results: Fifty four retinal whole mounts were available for analysis and scoring. Group 1 retinas had significantly higher values of vaso-obliteration, tufts, neovascularization, vessel tortuosity and higher mean retinopathy scores than Group 2 retinas (KO mice: 9.0±0.27 vs. 0.74±0.2, respectively, P<0.0001; WT mice: 7.58±0.40 vs. 1.17±0.27, respectively, P<0.0001). Manual scoring in Group 1 revealed higher values of neovascularization, tortuosity and mean retinopathy scores in KO mice vs. WT mice (9.0±0.27 vs. 7.58±0.40, respectively, P0.009). Digital scoring revealed a higher neovascularization score in KO mice as well (13.72±0.82% vs. 8.06±0.27%, P<0.0001). All mice had similar vaso-obliteration areas. There were no significant differences between KO and WT mice in Group 2. Conclusions: Absence of CD24 may have a deleterious effect on angiogenesis occurring in the second stage of ROP development, though its role in vessel obliteration during the first stage of ROP is probably limited.
KW - Angiogenesis
KW - CD24
KW - Oxygen-induced retinopathy
KW - Retinopathy of prematurity
UR - http://www.scopus.com/inward/record.url?scp=84861036440&partnerID=8YFLogxK
U2 - 10.3109/02713683.2011.647226
DO - 10.3109/02713683.2011.647226
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AN - SCOPUS:84861036440
SN - 0271-3683
VL - 37
SP - 532
EP - 539
JO - Current Eye Research
JF - Current Eye Research
IS - 6
ER -