TY - CHAP
T1 - Intrastriatal transplantation of mouse bone marrow-derived stem cells improves motor behavior in a mouse model of Parkinson's disease
AU - Offen, D.
AU - Barhum, Y.
AU - Levy, Y. S.
AU - Burshtein, A.
AU - Panet, H.
AU - Cherlow, T.
AU - Melamed, E.
PY - 2007
Y1 - 2007
N2 - Strategies of cell therapy for the treatment of Parkinson's disease (PD) are focused on replacing damaged neurons with cells to restore or improve function that is impaired due to cell population damage. In our studies, we used mesenchymal stromal cells (MSCs) from mouse bone marrow. Following our novel neuronal differentiation method, we found that the basic cellular phenotype changed to cells with neural morphology that express specific markers including those characteristic for dopaminergic neurons, such as tyrosine hydroxylase (TH). Intrastriatal transplantation of the differentiated MSCs in 6-hydroxydopamine-lesioned mice led to marked reduction in the amphetamine-induced rotations. Immunohistological analysis of the mice brains four months post transplantation, demonstrated that most of the transplanted cells survived in the striatum and expressed TH. Some of the TH positive cells migrated toward the substantia nigra. In conclusion, transplantation of bone marrow derived stem cells differentiated to dopaminergic-like cells, successfully improved behavior in an animal model of PD suggesting an accessible source of cells that may be used for autotransplantation in patient with PD.
AB - Strategies of cell therapy for the treatment of Parkinson's disease (PD) are focused on replacing damaged neurons with cells to restore or improve function that is impaired due to cell population damage. In our studies, we used mesenchymal stromal cells (MSCs) from mouse bone marrow. Following our novel neuronal differentiation method, we found that the basic cellular phenotype changed to cells with neural morphology that express specific markers including those characteristic for dopaminergic neurons, such as tyrosine hydroxylase (TH). Intrastriatal transplantation of the differentiated MSCs in 6-hydroxydopamine-lesioned mice led to marked reduction in the amphetamine-induced rotations. Immunohistological analysis of the mice brains four months post transplantation, demonstrated that most of the transplanted cells survived in the striatum and expressed TH. Some of the TH positive cells migrated toward the substantia nigra. In conclusion, transplantation of bone marrow derived stem cells differentiated to dopaminergic-like cells, successfully improved behavior in an animal model of PD suggesting an accessible source of cells that may be used for autotransplantation in patient with PD.
KW - Multipotent mesenchymal stromal cells (MSCs)
KW - Parkinson's disease (PD)
KW - dopamine
KW - dopaminergic neurons
KW - stem cells
UR - http://www.scopus.com/inward/record.url?scp=38449109612&partnerID=8YFLogxK
U2 - 10.1007/978-3-211-73574-9_16
DO - 10.1007/978-3-211-73574-9_16
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C2 - 17982886
AN - SCOPUS:38449109612
SN - 9783211735732
T3 - Journal of Neural Transmission, Supplementa
SP - 133
EP - 143
BT - Neuropsychiatric Disorders An Integrative Approach
PB - Springer Wien
ER -