Intracellular and intercellular movement of maize streak geminivirus V1 and V2 proteins transiently expressed as green fluorescent protein fusions

Guy Kotlizky, Margaret I. Boulton, Chetsadaporn Pitaksutheepong, Jeffrey W. Davies, Bernard L. Epel*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

50 Scopus citations

Abstract

Transient expression of the maize streak geminivirus virion-sense proteins V1 and V2 (movement protein, MP, and coat protein, CP, respectively) in maize leaves allowed investigation of their roles in inter- and intracellular movement. Bombardment of a construct directing expression of a V1:green fluorescent protein (GFP) fusion product resulted in significantly increased spread of fluorescence from the bombarded cell to adjacent cells compared to that obtained following expression of free GFP. A mutant V1:GFP fusion product exhibited markedly less movement than the V1:GFP protein. Thus, the MSV V1 protein moves from cell to cell in the absence of other viral proteins. However, V1:GFP did not localize to plasmodesmata in maize or tobacco leaves although a tobacco mosaic virus MP:GFP fusion protein was shown to do so in tobacco. The CP:GFP fusion product targeted exclusively to the nucleus and did not move from cell to cell or exit the nucleus when expressed alone. When coexpressed with V1, some CP:GFP fluorescence was seen at the cell periphery in a proportion of cells, but in no case was cell-to-cell movement of CP:GFP detected. The likely roles of V1 and CP in MSV movement are discussed. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)32-38
Number of pages7
JournalVirology
Volume274
Issue number1
DOIs
StatePublished - 15 Aug 2000

Funding

FundersFunder number
United States–Israel Research and Development FundIS 2636-95
Biotechnology and Biological Sciences Research Council

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