Intestinal alkaline phosphatase is secreted bidirectionally from villous enterocytes

N. L. Sussman, R. Eliakim, D. Rubin, D. H. Perlmutter, K. DeSchryver-Kecskemeti, D. H. Alpers

Research output: Contribution to journalArticlepeer-review

56 Scopus citations

Abstract

A fraction of intestinal alkaline phosphatase (IAP) is secreted into blood. To study this process, enzyme secretion was examined in a fetal (IRD-98) and a differentiated (Caco-2) intestinal cell line. Tissue-unspecific alkaline phosphatase (AP) activity in the IRD-98 cells increased 20-fold after addition of 1.5 mM sodium butyrate and 40 mM NaCl, but no AP activity was secreted into the medium. In contrast, newly synthesized IAP in Caco-2 cells was secreted into the medium. AP secretion increased with time and was inhibited by monensin. Medium AP was still partially bound to membranes as assessed by Triton X-114 phase separation and could be released by the addition of serum. Analysis by sodium dodecyl sulfate polyacrylamide gels and by isoelectric focussing showed that secreted AP gave a pattern similar to that of the AP released from membranes by phospholipase D treatment. When Caco-2 cells were grown on filters, AP activity was found in both basolateral (75%) and luminal (25%) media. These data demonstrate that the secretion of a particulate AP with extracellular release from the membrane can account for the appearance of the intestinal isozyme in both the serum and the lumen.

Original languageEnglish
Pages (from-to)20/1
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume257
Issue number1
StatePublished - 1989
Externally publishedYes

Fingerprint

Dive into the research topics of 'Intestinal alkaline phosphatase is secreted bidirectionally from villous enterocytes'. Together they form a unique fingerprint.

Cite this