Interleukin-33 requires CMRF35-like molecule-1 expression for induction of myeloid cell activation

D. Shik, I. Moshkovits, D. Karo-Atar, H. Reichman, A. Munitz*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Background IL-33 is a potent activator of various cells involved in allergic inflammation, including eosinophils and mast cells. Despite its critical role in Th2 disease settings, endogenous molecular mechanisms that may regulate IL-33-induced responses remain to be defined. We have recently shown that eosinophils express CMRF35-like molecule (CLM)-1. Yet, the role of CLM-1 in regulating eosinophil functions is still elusive. Methods CLM-1 and CLM-8 expression and cellular localization were assessed in murine bone marrow-derived and/or peritoneal cells at baseline and following IL-33 stimulation (flow cytometry, western blot). IL-33-induced mediator release and signaling were assessed in wild-type (wt) and Clm1-/- cells and mice. Results BM-derived eosinophils express high levels of glycosylated CLM-1. IL-33 induced a rapid, specific, concentration- and time-dependent upregulation of CLM-1 in eosinophils (in vitro and in vivo). Clm1-/- eosinophils secreted less IL-33-induced mediators than wt eosinophils. CLM-1 co-localized to ST2 following IL-33 stimulation and was required for IL-33-induced NFκB and p38 phosphorylation. Th2 cytokine (e.g., IL-5, IL-13) and chemokine (e.g., eotaxins, CCL2) secretion was markedly attenuated in IL-33-treated Clm1 -/- mice. Subsequently, IL-33-challenged mice displayed reduced infiltration of mast cells, macrophages, neutrophils, and B cells. Despite the markedly impaired IL-33-induced eotaxin expression in Clm1-/- mice, eosinophil accumulation was similar in wt and Clm1-/- mice, due to hyperchemotactic responses of Clm1-/- eosinophils. Conclusions CLM-1 is a novel regulator of IL-33-induced eosinophil activation. These data contribute to the understanding of endogenous molecular mechanisms regulating IL-33-induced responses and may ultimately lead to receptor-based tools for future therapeutic intervention in IL-33-associated diseases.

Original languageEnglish
Pages (from-to)719-729
Number of pages11
JournalAllergy: European Journal of Allergy and Clinical Immunology
Volume69
Issue number6
DOIs
StatePublished - Jun 2014

Keywords

  • CMRF-35-like molecule 1
  • IL-33
  • eosinophils

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