Interleukin-2 inhibits the replication of human herpesvirus-6 in mature thymocytes

Ehud Roffman; Niza Frenkel

doi:10.1016/0042-6822(90)90447-Y

Interleukin-2 inhibits the replication of human herpesvirus-6 in mature thymocytes

Ehud Roffman, Niza Frenkel^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

Human herpesvirus-6 (HHV-6) is a recently identified T lymphotropic virus. We have examined the ability of HHV-6 to replicate in mature and immature human thymocytes. Infection of both cell populations revealed that only mitogen-activated mature thymocytes could support efficient virus replication. Because interleukin-2 (IL-2) plays a central role in T cell activation we investigated its effect on HHV-6 replication. Unexpectedly, addition of recombinant IL-2 at concentrations exceeding 10 U/ml strongly inhibited the virus-induced cytopathic effect. Electron microscopic examinations and immunofluorescence assays revealed a threefold reduction in the fraction of infected cells, and almost total absence of extracellular virions in the IL-2-treated cultures. It will therefore be of interest to determine whether the IL-2-mediated inhibitory effect plays some role in the establishment of HHV-6 latency in the human host.

Original language	English
Pages (from-to)	591-594
Number of pages	4
Journal	Virology
Volume	175
Issue number	2
DOIs	https://doi.org/10.1016/0042-6822(90)90447-Y
State	Published - Apr 1990
Externally published	Yes

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title = "Interleukin-2 inhibits the replication of human herpesvirus-6 in mature thymocytes",

abstract = "Human herpesvirus-6 (HHV-6) is a recently identified T lymphotropic virus. We have examined the ability of HHV-6 to replicate in mature and immature human thymocytes. Infection of both cell populations revealed that only mitogen-activated mature thymocytes could support efficient virus replication. Because interleukin-2 (IL-2) plays a central role in T cell activation we investigated its effect on HHV-6 replication. Unexpectedly, addition of recombinant IL-2 at concentrations exceeding 10 U/ml strongly inhibited the virus-induced cytopathic effect. Electron microscopic examinations and immunofluorescence assays revealed a threefold reduction in the fraction of infected cells, and almost total absence of extracellular virions in the IL-2-treated cultures. It will therefore be of interest to determine whether the IL-2-mediated inhibitory effect plays some role in the establishment of HHV-6 latency in the human host.",

author = "Ehud Roffman and Niza Frenkel",

note = "Funding Information: for the use of the EM facility; and Dr. N. Balachandran (University 01 Kansas Medical Center) for the HHV-6 monoclonal antibody. The initial studies were supported by U.S. Public Health Service Research Grant All 5488 to The University of Chicago.",

year = "1990",

month = apr,

doi = "10.1016/0042-6822(90)90447-Y",

language = "אנגלית",

volume = "175",

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AU - Roffman, Ehud

AU - Frenkel, Niza

N1 - Funding Information: for the use of the EM facility; and Dr. N. Balachandran (University 01 Kansas Medical Center) for the HHV-6 monoclonal antibody. The initial studies were supported by U.S. Public Health Service Research Grant All 5488 to The University of Chicago.

PY - 1990/4

Y1 - 1990/4

N2 - Human herpesvirus-6 (HHV-6) is a recently identified T lymphotropic virus. We have examined the ability of HHV-6 to replicate in mature and immature human thymocytes. Infection of both cell populations revealed that only mitogen-activated mature thymocytes could support efficient virus replication. Because interleukin-2 (IL-2) plays a central role in T cell activation we investigated its effect on HHV-6 replication. Unexpectedly, addition of recombinant IL-2 at concentrations exceeding 10 U/ml strongly inhibited the virus-induced cytopathic effect. Electron microscopic examinations and immunofluorescence assays revealed a threefold reduction in the fraction of infected cells, and almost total absence of extracellular virions in the IL-2-treated cultures. It will therefore be of interest to determine whether the IL-2-mediated inhibitory effect plays some role in the establishment of HHV-6 latency in the human host.

AB - Human herpesvirus-6 (HHV-6) is a recently identified T lymphotropic virus. We have examined the ability of HHV-6 to replicate in mature and immature human thymocytes. Infection of both cell populations revealed that only mitogen-activated mature thymocytes could support efficient virus replication. Because interleukin-2 (IL-2) plays a central role in T cell activation we investigated its effect on HHV-6 replication. Unexpectedly, addition of recombinant IL-2 at concentrations exceeding 10 U/ml strongly inhibited the virus-induced cytopathic effect. Electron microscopic examinations and immunofluorescence assays revealed a threefold reduction in the fraction of infected cells, and almost total absence of extracellular virions in the IL-2-treated cultures. It will therefore be of interest to determine whether the IL-2-mediated inhibitory effect plays some role in the establishment of HHV-6 latency in the human host.

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Interleukin-2 inhibits the replication of human herpesvirus-6 in mature thymocytes

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