TY - JOUR
T1 - Interferons α and β down-regulate the expression of basic fibroblast growth factor in human carcinomas
AU - Singh, Rakesh K.
AU - Gutman, Mordechai
AU - Bucana, Corazon D.
AU - Sanchez, Ricardo
AU - Llansa, Norma
AU - Fidler, Isaiah J.
PY - 1995/5/9
Y1 - 1995/5/9
N2 - We investigated the influence of interferons α, β, and γ (IFN-α, -β, and -γ) on the production of basic fibroblast growth factor (bFGF) by human renal carcinoma cells. The human renal carcinoma cell metastatic line SN12PM6 was established in culture from a lung metastasis and SN12PM6-resistant cells were selected in vitro for resistance to the antiproliferative effects of IFN-α or IFN-β. IFN-α and IFN-β, but not IFN-γ, down-regulated the expression of bFGF at the mRNA and protein levels by a mechanism independent of their antiproliferative effects. Downregulation of bFGF required a long exposure (>4 days) of cells to low concentrations (> 10 units/ml) of IFN-α or IFN-β. The withdrawal of IFN-α or IFN-β from the medium permitted SN12PM6-resistant cells to resume production of bFGF. The incubation of human bladder, prostate, colon, and breast carcinoma cells with noncytostatic concentrations of IFN-α or IFN-β also produced down-regulation of bFGF production.
AB - We investigated the influence of interferons α, β, and γ (IFN-α, -β, and -γ) on the production of basic fibroblast growth factor (bFGF) by human renal carcinoma cells. The human renal carcinoma cell metastatic line SN12PM6 was established in culture from a lung metastasis and SN12PM6-resistant cells were selected in vitro for resistance to the antiproliferative effects of IFN-α or IFN-β. IFN-α and IFN-β, but not IFN-γ, down-regulated the expression of bFGF at the mRNA and protein levels by a mechanism independent of their antiproliferative effects. Downregulation of bFGF required a long exposure (>4 days) of cells to low concentrations (> 10 units/ml) of IFN-α or IFN-β. The withdrawal of IFN-α or IFN-β from the medium permitted SN12PM6-resistant cells to resume production of bFGF. The incubation of human bladder, prostate, colon, and breast carcinoma cells with noncytostatic concentrations of IFN-α or IFN-β also produced down-regulation of bFGF production.
KW - angiogenesis
UR - http://www.scopus.com/inward/record.url?scp=0029075603&partnerID=8YFLogxK
U2 - 10.1073/pnas.92.10.4562
DO - 10.1073/pnas.92.10.4562
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C2 - 7753843
AN - SCOPUS:0029075603
SN - 0027-8424
VL - 92
SP - 4562
EP - 4566
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 10
ER -
