Interferon-dependent induction of mRNA activity for (2′-5′) oligo-isoadenylate synthetase

Lester Shulman*, Michel Revel

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


At least three different enzymes involved in the regulation of protein synthesis are induced in a variety of cells by interferon (IFN)1-5. Sensitive assays for these enzymes have been developed and used to establish the specificity, dose dependence and time course of their induction by IFN 5-8. One of these enzymes, the oligo-isoadenylate synthetase E, whose product (2′-5′)pppApApA9 activates the latent ribonuclease F10-11, is increased over 50-fold after IFN treatment. We describe here the assay for an mRNA from IFN-treated mouse L cells, that produces oligo-isoadenylate synthetase activity when injected into Xenopus oocytes. This mRNA is found in the cells only after exposure to IFN. The mRNA increases in mouse L cells with the same time course as the enzyme activity itself. In particular, there is a 3-h lag period between IFN addition and the onset of enzyme and mRNA accumulation. Using anti-IFN antibodies, we show that during this lag period the continued interaction of IFN with the cells is necessary for the full induction of the oligo-isoadenylate synthetase.

Original languageEnglish
Pages (from-to)98-100
Number of pages3
Issue number5786
StatePublished - 1980
Externally publishedYes


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