TY - JOUR
T1 - Interferon abrogates the arrest of DNA synthesis in heterologous thymocytes treated with lectins
AU - Sela, Ben Ami
AU - Gurari-Rotman, Dalia
N1 - Funding Information:
We thank Drs. Nathan Sharon and Michel Revel for their interest and encouragement. The work was supported by grants from the Israel Cancer Research Fund Inc. (Ne w York) and from the Bat-Sheva de Rothschild Foundation for the Advancement of Science in Israel.
PY - 1978/10/16
Y1 - 1978/10/16
N2 - Mouse or rat thymocytes are triggered to undergo blastogenesis by mitogenic doses of concanavalin A and lentil lectin, when these plant mitogens are applied to the freshly cultured thymocytes at time 0. However, these mitogens did not elicit a mitogenic response if added to mouse or rat thymocytes that were preincubated in culture medium for 24 hours. Incubation of the thymocytes with heterologous preparations of interferon during the period of 24 hours before application of the mitogens, brought about an enhanced incorporation of tritiated thymidine. The data presented suggest that heterologous interferons could significantly abrogate the block in DNA synthesis in thymocytes that were preincubated for 24 hours in culture medium prior to addition of the mitogens.
AB - Mouse or rat thymocytes are triggered to undergo blastogenesis by mitogenic doses of concanavalin A and lentil lectin, when these plant mitogens are applied to the freshly cultured thymocytes at time 0. However, these mitogens did not elicit a mitogenic response if added to mouse or rat thymocytes that were preincubated in culture medium for 24 hours. Incubation of the thymocytes with heterologous preparations of interferon during the period of 24 hours before application of the mitogens, brought about an enhanced incorporation of tritiated thymidine. The data presented suggest that heterologous interferons could significantly abrogate the block in DNA synthesis in thymocytes that were preincubated for 24 hours in culture medium prior to addition of the mitogens.
UR - http://www.scopus.com/inward/record.url?scp=0018087866&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(78)90741-6
DO - 10.1016/0006-291X(78)90741-6
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AN - SCOPUS:0018087866
SN - 0006-291X
VL - 84
SP - 550
EP - 556
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -