Interferon-β-related DNA is dispersed in the human genome

Anurag D. Sagar; Pravinkumar B. Sehgal; Lester T. May; Masayori Inouye; Doris L. Slate; Lester Shulman; Frank H. Ruddle

doi:10.1126/science.6546621

Interferon-β-related DNA is dispersed in the human genome

Anurag D. Sagar, Pravinkumar B. Sehgal^*, Lester T. May, Masayori Inouye, Doris L. Slate, Lester Shulman, Frank H. Ruddle

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Interferon-β₁ (IFN-β₁) complementary DNA was used as a hybridization probe to isolate human genomic DNA clones λB3 and λB4 from a human genomic DNA library. Blot-hybridization procedures and partial nucleotide sequencing revealed that λB3 is related to IFN-β₁ (and more distantly to IFN-α₁). Analyses of DNA obtained from a panel of human-rodent somatic cell hybrids that were probed with DNA derived from λB3 showed that λB3 is on human chromosome 2. Similar experiments indicated that λB4 is not on human chromosomes 2, 5, or 9. The finding that DNA related to the IFN- β₁ gene (and IFN-α₁ gene) is dispersed in the human genome raises new questions about the origins of the interferon genes.

Original language	English
Pages (from-to)	1312-1315
Number of pages	4
Journal	Science
Volume	223
Issue number	4642
DOIs	https://doi.org/10.1126/science.6546621
State	Published - 1984
Externally published	Yes

Funding

Funders	Funder number
National Institute of Allergy and Infectious Diseases	R01AI016262

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title = "Interferon-β-related DNA is dispersed in the human genome",

abstract = "Interferon-β1 (IFN-β1) complementary DNA was used as a hybridization probe to isolate human genomic DNA clones λB3 and λB4 from a human genomic DNA library. Blot-hybridization procedures and partial nucleotide sequencing revealed that λB3 is related to IFN-β1 (and more distantly to IFN-α1). Analyses of DNA obtained from a panel of human-rodent somatic cell hybrids that were probed with DNA derived from λB3 showed that λB3 is on human chromosome 2. Similar experiments indicated that λB4 is not on human chromosomes 2, 5, or 9. The finding that DNA related to the IFN- β1 gene (and IFN-α1 gene) is dispersed in the human genome raises new questions about the origins of the interferon genes.",

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AU - Sagar, Anurag D.

AU - Sehgal, Pravinkumar B.

AU - May, Lester T.

AU - Inouye, Masayori

AU - Slate, Doris L.

AU - Shulman, Lester

AU - Ruddle, Frank H.

PY - 1984

Y1 - 1984

N2 - Interferon-β1 (IFN-β1) complementary DNA was used as a hybridization probe to isolate human genomic DNA clones λB3 and λB4 from a human genomic DNA library. Blot-hybridization procedures and partial nucleotide sequencing revealed that λB3 is related to IFN-β1 (and more distantly to IFN-α1). Analyses of DNA obtained from a panel of human-rodent somatic cell hybrids that were probed with DNA derived from λB3 showed that λB3 is on human chromosome 2. Similar experiments indicated that λB4 is not on human chromosomes 2, 5, or 9. The finding that DNA related to the IFN- β1 gene (and IFN-α1 gene) is dispersed in the human genome raises new questions about the origins of the interferon genes.

AB - Interferon-β1 (IFN-β1) complementary DNA was used as a hybridization probe to isolate human genomic DNA clones λB3 and λB4 from a human genomic DNA library. Blot-hybridization procedures and partial nucleotide sequencing revealed that λB3 is related to IFN-β1 (and more distantly to IFN-α1). Analyses of DNA obtained from a panel of human-rodent somatic cell hybrids that were probed with DNA derived from λB3 showed that λB3 is on human chromosome 2. Similar experiments indicated that λB4 is not on human chromosomes 2, 5, or 9. The finding that DNA related to the IFN- β1 gene (and IFN-α1 gene) is dispersed in the human genome raises new questions about the origins of the interferon genes.

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Interferon-β-related DNA is dispersed in the human genome

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