Interference of cranberry constituents in cell-cell signaling system of vibrio harveyi

Mark Feldman, Ervin I. Weiss, Itzhak Ofek, Doron Steinberg

Research output: Contribution to journalArticlepeer-review

Abstract

Cranberry juice has long been recognized in folk medicine as a therapeutic agent, mainly in urinary track infections. It acts as an antibiofilm agent against various pathogens. Quorum sensing is process where bacteria communicate with each other via signal molecules known as autoinducers. This process is strongly involved in various bacterial pathological and physiological pathways. Various strains of Vibrio harveyi bacteria were incubated with different concentrations of nondialyzable material of cranberry (NDM) with or without addition of exogenous autoinducer. Bioluminescence regulated by the autoinducers was measured in GENios reader. Effect of NDM alone or NDM supplemented with autoinducer on quorum sensing was determined as change in bioluminescence in each treated sample compared to appropriate control in every strain. Using model of V. harveyi, we found an inhibitory effect of cranberry constituents on bacterial signaling system. This effect was reversible, since exogenous autoinducer was able to recover bioluminescence which was decreased by NDM. We hypothesized that cranberry NDM interacts with V. harveyi quorum sensing by competition with autoinducer for binding to autoinducer sensor.

Original languageEnglish
Pages (from-to)469-474
Number of pages6
JournalCurrent Microbiology
Volume59
Issue number4
DOIs
StatePublished - Oct 2009

Fingerprint

Dive into the research topics of 'Interference of cranberry constituents in cell-cell signaling system of vibrio harveyi'. Together they form a unique fingerprint.

Cite this