TY - JOUR
T1 - Interaction of prostaglandins with blood plasma proteins. I. Binding of prostaglandin E2 to human plasma proteins and its effect on the physiological activity of prostaglandin E2 in vitro and in vivo
AU - Raz, Amiram
PY - 1972/12/8
Y1 - 1972/12/8
N2 - 1. 1. The binding of prostaglandin E2 to human plasma proteins was investigated by DEAE-Sephadex column chromatography and acrylamide gel electrophoresis, and quantitatively assessed by equilibrium dialysis. Prostaglandin E2; added to human plasma in vitro was found to become mainly bound to plasma albumin. This binding was also demonstrated by adding prostaglandin E2 to human serum albumin solutions. 2. 2. The binding of prostaglandin E2 to human serum albumin inhibits the contraction producing effect of prostaglandin E2 on the isolated gerbil colon in vitro. 3. 3. The depressor effect of prostaglandin E2 on the rat blood pressure was used to assess the in vivo effect of prostaglandin E2-albumin interaction. The blood pressure lowering activities of free and albumin-bound prostaglandin E2 were found to be the same when administered either intravenously or intra-arterially. 4. 4. The significance of these observations with regard to estimation of prostaglandin concentration in whole blood or plasma, and their possible effects on prostaglandin metabolism is discussed.
AB - 1. 1. The binding of prostaglandin E2 to human plasma proteins was investigated by DEAE-Sephadex column chromatography and acrylamide gel electrophoresis, and quantitatively assessed by equilibrium dialysis. Prostaglandin E2; added to human plasma in vitro was found to become mainly bound to plasma albumin. This binding was also demonstrated by adding prostaglandin E2 to human serum albumin solutions. 2. 2. The binding of prostaglandin E2 to human serum albumin inhibits the contraction producing effect of prostaglandin E2 on the isolated gerbil colon in vitro. 3. 3. The depressor effect of prostaglandin E2 on the rat blood pressure was used to assess the in vivo effect of prostaglandin E2-albumin interaction. The blood pressure lowering activities of free and albumin-bound prostaglandin E2 were found to be the same when administered either intravenously or intra-arterially. 4. 4. The significance of these observations with regard to estimation of prostaglandin concentration in whole blood or plasma, and their possible effects on prostaglandin metabolism is discussed.
UR - http://www.scopus.com/inward/record.url?scp=0015498636&partnerID=8YFLogxK
U2 - 10.1016/0005-2760(72)90140-3
DO - 10.1016/0005-2760(72)90140-3
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AN - SCOPUS:0015498636
SN - 0005-2760
VL - 280
SP - 602
EP - 613
JO - Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
JF - Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
IS - 4
ER -