Interaction of hyaluronic acid-linked phosphatidylethanolamine (HyPE) with LDL and its effect on the susceptibility of LDL lipids to oxidation

Edit Schnitzer, Arie Dagan, Miron Krimsky, Dov Lichtenberg, Ilya Pinchuk, Hadassa Shinar, Saul Yedgar

Research output: Contribution to journalArticlepeer-review

Abstract

The amphiphilic polysaccharide hyaluronic acid-linked phosphatidylethanolamine (HyPE), synthesized by covalently binding dipalmitoyl-phosphatidylethanolamine (DPPE) to short chain hyaluronic acid (mol. wt. ≃ 30 000), interacts with low-density lipoproteins (LDL), to form a 'sugar-decoration' of the LDL surface. This results in an increase in the apparent size of the LDL particles, as studied by photon correlation spectroscopy, and in broadening of the 1H NMR signals of the LDL's phospholipids. Experiments conducted with fluorescently-labeled HyPE indicate that the interaction of HyPE with LDL involves incorporation of the hydrocarbon chains of this amphiphilic polysaccharide into the outer monolayer of the LDL. This interaction also inhibits the copper-induced oxidation of the LDL polyunsaturated fatty acids, avoiding oxidation altogether when the concentration of HyPE is higher than a tenth of the concentration of the LDL's phospholipids. This can not be attributed to competitive binding of copper by HyPE. We propose that the protection of LDL lipids against copper-induced oxidation is due to formation of a sugar network around the LDL.

Original languageEnglish
Pages (from-to)149-160
Number of pages12
JournalChemistry and Physics of Lipids
Volume104
Issue number2
DOIs
StatePublished - Feb 2000

Keywords

  • Hyaluronic acid
  • Hyaluronic acid- linked phosphatidylethanolamine
  • Low density lipoprotein
  • Peroxidation

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