TY - JOUR
T1 - Interaction of FIE, a Polycomb protein, with pRb
T2 - A possible mechanism regulating endosperm development
AU - Mosquna, A.
AU - Katz, A.
AU - Shochat, S.
AU - Grafi, G.
AU - Ohad, N.
N1 - Funding Information:
Acknowledgements We acknowledge Drs. Leor Williams and Ofra Lotan for suggestions. This research was supported by grants awarded to N.O. by the Israel Sciences Foundation (Grant No. 503/00) and Grant No. IS-3158-99c from BARD, the USA-Israel Binational Agricultural Research and Development Fund
PY - 2004/7
Y1 - 2004/7
N2 - Inactivation of the Arabidopsis protein FERTILIZATION INDEPENDENT ENDOSPERM (FIE) induces division of the central cell of the embryo sac, leading to endosperm development in the absence of fertilization. The mechanism whereby FIE regulates this process is unknown. We postulated that activation of central cell division in fie mutant plants might involve the retinoblastoma protein (pRb), a cell cycle regulatory element. Pull-down and surface plasmon resonance assays demonstrated that FIE interacts in-vitro with the pRb homologues from Arabidopsis (AtRb), maize (ZmRb) and human (HuRb). The interaction of FIE with ZmRB and HuRb in the yeast two-hybrid system supports the possibility that a FIE-pRb interaction may occur also in planta. Mutational analysis showed that this interaction does not occur via the LxCxE motif of the FIE protein nor via the pocket B domain of pRb. These results suggest that FIE may inhibit premature division of the central cell of the embryo sac, at least partly, through interaction with pRb, and suppression of pRb-regulated genes.
AB - Inactivation of the Arabidopsis protein FERTILIZATION INDEPENDENT ENDOSPERM (FIE) induces division of the central cell of the embryo sac, leading to endosperm development in the absence of fertilization. The mechanism whereby FIE regulates this process is unknown. We postulated that activation of central cell division in fie mutant plants might involve the retinoblastoma protein (pRb), a cell cycle regulatory element. Pull-down and surface plasmon resonance assays demonstrated that FIE interacts in-vitro with the pRb homologues from Arabidopsis (AtRb), maize (ZmRb) and human (HuRb). The interaction of FIE with ZmRB and HuRb in the yeast two-hybrid system supports the possibility that a FIE-pRb interaction may occur also in planta. Mutational analysis showed that this interaction does not occur via the LxCxE motif of the FIE protein nor via the pocket B domain of pRb. These results suggest that FIE may inhibit premature division of the central cell of the embryo sac, at least partly, through interaction with pRb, and suppression of pRb-regulated genes.
KW - Arabidopsis thaliana
KW - Cell cycle regulation
KW - Central cell of the embryo sac
KW - Polycomb proteins
KW - Retinoblastoma
UR - http://www.scopus.com/inward/record.url?scp=4043052454&partnerID=8YFLogxK
U2 - 10.1007/s00438-004-1024-6
DO - 10.1007/s00438-004-1024-6
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AN - SCOPUS:4043052454
SN - 1617-4615
VL - 271
SP - 651
EP - 657
JO - Molecular Genetics and Genomics
JF - Molecular Genetics and Genomics
IS - 6
ER -
