Inter- and intra-lesional molecular heterogeneity of oral leukoplakia

Carolina Cavalieri Gomes*, Thiago Fonseca-Silva, Clarice Ferreira Galvão, Eitan Friedman, Luiz De Marco, Ricardo Santiago Gomez

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Objectives: Oral leukoplakia (OL) is the most common potentially malignant lesion of the oral cavity, and OL diagnosis is a risk factor for developing subsequent oral squamous cell carcinomas (OSCC). Notably, loss of heterozygosity (LOH) profiles have been validated as risk predictors of malignant transformation of OL. Similar to other solid malignant tumors, OSCC exhibit molecular heterogeneity. However, if and to what extent tumor heterogeneity is present in premalignant lesions of the oral cavity has not been addressed. As LOH analysis is currently being used to stratify OL patients at risk for OSCC development in chemoprevention studies, insight into the issue of molecular heterogeneity of OL is of clinical significance. Materials and methods: To address this issue, 11 polymorphic microsatellite markers localizing to chromosomes 3, 9, 11 and 17 were used to detect LOH in 28 samples of 14 OL patients, by capillary electrophoresis analysis. These samples were either clinically recurrent lesions, or two anatomically distinct biopsies from the same lesion, or even two different OL lesions located at distinct intraoral sites. Results: In all but one of the biopsies pairs, distinct LOH patterns were displayed regardless of histopathological grade. These data provide evidence for inter- and intra-lesional molecular heterogeneity in OL. Conclusions: On the basis of these findings, molecular heterogeneity needs to be addressed in subsequent studies targeting specific carcinogenic pathways/genes in chemoprevention of malignant transformation of OL.

Original languageEnglish
Pages (from-to)178-181
Number of pages4
JournalOral Oncology
Volume51
Issue number2
DOIs
StatePublished - 2015

Keywords

  • 3p
  • 9p
  • LOH, loss of heterozygosity
  • Oral cancer
  • Oral dysplasia
  • Potentially malignant oral disorders

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