Integration of automated morphological features resolves a distinct group of atypical chronic lymphocytic leukemias with chromosomal aberrations

Yair Herishanu, Sigi Kay, Erel Joffe, Jonathan Ben-Ezra, Shoshana Baron, Rachel Rotman, Rony Braunstein, Nili Dezorella, Aaron Polliack, Elizabeth Naparstek, Chava Perry, Varda Deutsch, Ben Zion Katz

Research output: Contribution to journalArticlepeer-review

Abstract

Automated morphological assessment of peripheral blood slides has become an important modality facilitating characterization and quantification of cells in a uniform, fast and robust manner. In this study, we evaluated the morphological diversity in peripheral blood films of 94 chronic lymphocytic leukemia (CLL) patients using the DM1200 CellaVision automated microscopy system. Aberrant lymphocytes and smudge cells were enumerated and correlated with CLL immunophenotype, chromosomal aberrations and prognostic parameters. Herein, we show that the percentages of aberrant and smudge cells was highly variable between patients and did not correlate with each other. Increased aberrant lymphocytes and fewer smudge cells were associated with an atypical immunophenotype including low expression of CD23, higher levels of FMC7 and bright surface levels of CD20. High fraction of aberrant lymphocytes also was associated with trisomy 12. These cells were predominantly of small/medium size, sometimes with cleft nuclei. No correlation was noted between aberrant or smudge cells and clinical stage, CD38, ZA70 or time to first treatment. Taken together, automated morphological analysis of peripheral blood leukocytes emerged as a powerful and robust tool for the quantitative morphological stratification of CLL. Integration of the automated morphological features discriminates between different CLL phenotypes and distinct chromosomal aberrations.

Original languageEnglish
Pages (from-to)484-489
Number of pages6
JournalLeukemia Research
Volume38
Issue number4
DOIs
StatePublished - Apr 2014

Keywords

  • Aberrant morphology
  • CLL
  • Chromosomal abnormalities
  • Peripheral blood

Fingerprint

Dive into the research topics of 'Integration of automated morphological features resolves a distinct group of atypical chronic lymphocytic leukemias with chromosomal aberrations'. Together they form a unique fingerprint.

Cite this