Insulin-like growth factor-1 inhibits cell death induced by anticancer drugs in the MCF-7 cells: Involvement of growth factors in drug resistance

Avraham Geier*, Rachel Beery, Michal Haimsohn, Avraham Karasik

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The involvement of growth factors in cell survival in the presence of anticancer drugs was investigated. Cell death was induced in the human breast cancer cell line MCF-7, by the structurally and mechanistically unrelated chemotherapeutic drugs puromycin, actinomycin D, 5-fluorouracil, cisplatin, and adriamycin. The effect of insulin-like growth factor-1 (IGF-1), epidermal growth factor (EGF), and insulin on cell death was evaluated by two different methods: (1) trypan blue dye exclusion test and (2) lactic dehydrogenase release into the culture medium. IGF-1 inhibited cell death induced by each of the diverse drugs in a concentration-dependent manner reaching a maximal effect at 40 ng/ml. Insulin mimicked the effect of IGF-1 only at supraphysiological concentration with an optimal effect at 10,000 ng/ml. EGF had no effect on cell death up to 100 ng/ml. Our finding that IGF-1 specifically enhanced MCF-7 cell survival in the presence of different anticancer drugs suggests the involvement of growth factors in the mechanism of drug resistance.

Original languageEnglish
Pages (from-to)480-486
Number of pages7
JournalCancer Investigation
Volume13
Issue number5
DOIs
StatePublished - 1995
Externally publishedYes

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