Insulin-Induced Hypertension, L-Arginine, and Endothelial Nitric Oxide Synthase in Pregnant Rats

Michael Bursztyn, Eduardo Podjarny, Rachel Dahan, Itamar Raz, Jacques Bernheim

Research output: Contribution to journalArticlepeer-review

Abstract

Pregnancy-induced hypertension in rats with chronic exogenous hyperinsulinemia is associated with reduced urinary excretion of nitric oxide metabolites. We tested the hypothesis that there are perturbations of endothelial nitric oxide synthase in their kidneys. We studied three groups of rats: control pregnant rats (n = 6); pregnant rats with hyperinsulinemia by subcutaneous sustained-release insulin pellet (n = 5); and hyperinsulinemic pregnant rats treated with L-arginine 2 gL in drinking water (n = 5). By the end of pregnancy blood pressure was 78 ± 12 mm Hg in controls, 119 ± 15 mm Hg in hyperinsulinemic rats, and 77 ± 8 mm Hg in L-arginine-treated hyperinsulinemic rats, p < 0.007. Serum creatinine was 0. 4 mg/dl in controls, 0.6 mg/dl in hyperinsulinemic rats, and 0.5 mg/dl in L-arginine-treated rats, p < 0.05. Corresponding urinary excretion of nitric oxide metabolites was 2.1 ± 0.5, 1.2 ± 0.2, and 1.5 ± 0.2 μmols/mg creatinine, p < 0.01. Expression of endothelial nitric oxide synthase protein in kidneys by Western blot was not different between controls and hyperinsulinemic rats, 5.6 ± 2.4 and 5.8 ± 3.4 OD x mm 2, but was nearly doubled in L-arginine-treated rats, 10.8 ± 2.3, p < 0.03. Thus, the salutary effect of L-arginine on hyperinsulinemic pregnancy-induced hypertension (PIH) may be mediated, in part, by endothelial nitric oxide synthase in their kidneys.

Original languageEnglish
Pages (from-to)267-274
Number of pages8
JournalHypertension in Pregnancy
Volume22
Issue number3
DOIs
StatePublished - 2003
Externally publishedYes

Keywords

  • Blood pressure
  • Hyperinsulinemia
  • Intrauterine growth restriction
  • Pregnancy-induced hypertension

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