Insulin and fatty acids regulate the expression of the fat droplet-associated protein adipophilin in primary human trophoblasts

Uriel Elchalal*, W. Timothy Schaiff, Steven D. Smith, Eli Rimon, Ibrahim Bildirici, D. Michael Nelson, Yoel Sadovsky

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Objective: This study was undertaken to test the hypothesis that insulin and fatty acids regulate adipophilin expression in cultured human trophoblasts. Study design: Cytotrophoblasts isolated from term human placentas were cultured in the absence or presence of insulin (10 nmol/L), and a mix of oleic and linoleic acid in serum-free medium. The expression of adipophilin as well as the fatty acid transport proteins (FATP) 2, 3, 4 and 6 was examined. Fat accumulation was quantified by BODIPY staining and fat uptake determined using [3H]-oleic acid. Results: A combination of insulin and fatty acids enhanced the expression of adipophilin (2.3-fold, P < .05). In contrast, the expression of FATPs was unchanged. Furthermore, insulin and fatty acids increased the accumulation of fat droplets in trophoblasts by 4- to 5-fold (P < .05), but had no effect on oleic acid uptake. Conclusion: Insulin and fatty acids enhance the expression of adipophilin and the formation of fatty acid droplets in term human trophoblasts.

Original languageEnglish
Pages (from-to)1716-1723
Number of pages8
JournalAmerican Journal of Obstetrics and Gynecology
Volume193
Issue number5
DOIs
StatePublished - Nov 2005
Externally publishedYes

Funding

FundersFunder number
National Institutes of HealthHD-45671, ES-11597
Eunice Kennedy Shriver National Institute of Child Health and Human DevelopmentR01HD029190

    Keywords

    • Adipophilin
    • Fatty acids
    • Insulin
    • Placenta
    • Trophoblast

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