@article{db72d319e4134eeba47e4d4db18e2db9,
title = "Insulin and fatty acids regulate the expression of the fat droplet-associated protein adipophilin in primary human trophoblasts",
abstract = "Objective: This study was undertaken to test the hypothesis that insulin and fatty acids regulate adipophilin expression in cultured human trophoblasts. Study design: Cytotrophoblasts isolated from term human placentas were cultured in the absence or presence of insulin (10 nmol/L), and a mix of oleic and linoleic acid in serum-free medium. The expression of adipophilin as well as the fatty acid transport proteins (FATP) 2, 3, 4 and 6 was examined. Fat accumulation was quantified by BODIPY staining and fat uptake determined using [3H]-oleic acid. Results: A combination of insulin and fatty acids enhanced the expression of adipophilin (2.3-fold, P < .05). In contrast, the expression of FATPs was unchanged. Furthermore, insulin and fatty acids increased the accumulation of fat droplets in trophoblasts by 4- to 5-fold (P < .05), but had no effect on oleic acid uptake. Conclusion: Insulin and fatty acids enhance the expression of adipophilin and the formation of fatty acid droplets in term human trophoblasts.",
keywords = "Adipophilin, Fatty acids, Insulin, Placenta, Trophoblast",
author = "Uriel Elchalal and Schaiff, {W. Timothy} and Smith, {Steven D.} and Eli Rimon and Ibrahim Bildirici and Nelson, {D. Michael} and Yoel Sadovsky",
note = "Funding Information: Supported by NIH ES-11597 and HD-45671 (both to Y.S.), and HD-29190 (D.M.N.). ",
year = "2005",
month = nov,
doi = "10.1016/j.ajog.2005.04.012",
language = "אנגלית",
volume = "193",
pages = "1716--1723",
journal = "American Journal of Obstetrics and Gynecology",
issn = "0002-9378",
publisher = "Elsevier Inc.",
number = "5",
}