@article{0fa986132ae64b7eb40b05ffbdf02a95,
title = "Insights into enhanced thermostability of a cellulosomal enzyme",
abstract = "Improved stability of cellulosomal enzymes is of great significance in order to provide efficient degradation of cellulosic derivatives for production of biofuels. In previous reports, we created a quadruple mutant of the endoglucanase Cel8A from Clostridium thermocellum resulting from a combination of both random error-prone PCR and a bioinformatics-based consensus mutagenesis approach. The quadruple mutant exhibited an increased half-life of activity by 14-fold at 85 °C with no apparent loss of catalytic activity compared to the wild-type form. Connection of the wild-type enzyme to its respective cohesin partner conferred increased thermostability, but no increase was observed for the cohesin-complexed mutant enzyme. The mutant and the wild-type enzymes were integrated into divalent chimeric scaffoldins with a family 48 exoglucanase partner, and the cellulose-degradation activities of resultant designer cellulosomes were examined. Despite the heightened thermostability of the mutant as a free enzyme, its substitution for the wild-type endoglucanase within the cellulosome context failed to exhibit an improvement in overall degradation of cellulose.",
keywords = "Cellulosome, Cohesin, Dockerin, Endoglucanase, Exoglucanase, Thermostability",
author = "Johanna Stern and Michael Anbar and Sarah Mora{\"i}s and Raphael Lamed and Bayer, \{Edward A.\}",
note = "Funding Information: The authors appreciate the advice and assistance of Dr. Yoav Barak of the Chemical and Biophysical NanoSciences Unit, Department of Chemical Research Support of the Weizmann Institute of Science. This research was supported by the F. Warren Hellman Grant for Alternative Energy Research in Israel in support of alternative energy research in Israel to E.A.B. administered by the Israel Strategic Alternative Energy Foundation (I-SAEF ). Additional support was obtained by a Grant (No. 24/11 ) issued to R.L. by The Sidney E. Frank Foundation through the Israel Science Foundation (ISF) and by a Grant (No. 1349 ) to E.A.B. also from the ISF . This research was also supported by the Establishment of an Israeli Center of Research Excellence I-CORE Center No. 152/11 ) managed by the Israel Science Foundation, by the Weizmann Institute of Science Alternative Energy Research Initiative (AERI) and the Helmsley Foundation (the Leona M. and Harry B. Helmsley Charitable Trust) , and Grants from the United States-Israel Binational Science Foundation (BSF) , Jerusalem, Israel. E.A.B. is the incumbent of The Maynard I. and Elaine Wishner Chair of Bio-organic Chemistry.",
year = "2014",
month = may,
day = "7",
doi = "10.1016/j.carres.2014.01.014",
language = "אנגלית",
volume = "389",
pages = "78--84",
journal = "Carbohydrate Research",
issn = "0008-6215",
publisher = "Elsevier Ltd.",
number = "1",
}
