Inositol lipids and phosphatidic acid inhibit cell-free activation of neutrophil NADPH oxidase

Irit Aviram*, Michaela Sharabani

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The effect of inositol lipids on the SDS-initiated cell-free activation of NADPH oxidase in membranes of human neutrophils was investigated. In a system consisting of low density membranes, cytosol and SDS, low doses of phosphatidylinositol, phosphatidylinositol mono- and biphosphates and phosphatidic acid interfered with activation of the oxidase. The inhibition was relieved by increasing concentrations of the cytosol. Conversely, preincubation of multilamellar phosphoinositide vesicles with cytosol reduced its ability to support activation of the oxidase.

Original languageEnglish
Pages (from-to)712-719
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume161
Issue number2
DOIs
StatePublished - 15 Jun 1989

Fingerprint

Dive into the research topics of 'Inositol lipids and phosphatidic acid inhibit cell-free activation of neutrophil NADPH oxidase'. Together they form a unique fingerprint.

Cite this