TY - JOUR
T1 - Inositol lipids and phosphatidic acid inhibit cell-free activation of neutrophil NADPH oxidase
AU - Aviram, Irit
AU - Sharabani, Michaela
PY - 1989/6/15
Y1 - 1989/6/15
N2 - The effect of inositol lipids on the SDS-initiated cell-free activation of NADPH oxidase in membranes of human neutrophils was investigated. In a system consisting of low density membranes, cytosol and SDS, low doses of phosphatidylinositol, phosphatidylinositol mono- and biphosphates and phosphatidic acid interfered with activation of the oxidase. The inhibition was relieved by increasing concentrations of the cytosol. Conversely, preincubation of multilamellar phosphoinositide vesicles with cytosol reduced its ability to support activation of the oxidase.
AB - The effect of inositol lipids on the SDS-initiated cell-free activation of NADPH oxidase in membranes of human neutrophils was investigated. In a system consisting of low density membranes, cytosol and SDS, low doses of phosphatidylinositol, phosphatidylinositol mono- and biphosphates and phosphatidic acid interfered with activation of the oxidase. The inhibition was relieved by increasing concentrations of the cytosol. Conversely, preincubation of multilamellar phosphoinositide vesicles with cytosol reduced its ability to support activation of the oxidase.
UR - http://www.scopus.com/inward/record.url?scp=0024384460&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(89)92658-2
DO - 10.1016/0006-291X(89)92658-2
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AN - SCOPUS:0024384460
SN - 0006-291X
VL - 161
SP - 712
EP - 719
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -