TY - JOUR
T1 - Inhibitory effect of monoclonal antibodies on Alzheimer's β-amyloid peptide aggregation
AU - Hanan, Eilat
AU - Solomon, Beka
PY - 1996
Y1 - 1996
N2 - In vitro aggregation of β-amyloid peptide induced by incubating the peptide for 3 h at 37°C, was investigated via immunocomplexation with a panel of monoclonal antibodies raised against various β-amyloid fragments. Some of these monoclonal antibodies prevented the formation of β-amyloid and this effect may be related to the localization of the antibody binding sites. Monoclonal antibodies 6C6 and 10D5, and AMY-33, which recognize epitopes spanning the amino acid residues 1-28 of β-amyloid peptide, inhibited its aggregation by 40%-90% when compared with aggregation occurring in the absence of the respective antibodies. The antibodies, 2H3 and 1C2, directed to the regions comprising peptides 1-12 and 13-28, respectively, had a considerably low effect. Identifying the "aggregating epitopes" as sequences that are related to the sites where protein aggregation is initiated and preparing monoclonal antibodies against these regions may facilitate the understanding and prevention of protein aggregation processes. This study may provide a factual basis for using monoclonal antibodies to prevent the β-amyloid formation that is associated with Alzheimer's disease.
AB - In vitro aggregation of β-amyloid peptide induced by incubating the peptide for 3 h at 37°C, was investigated via immunocomplexation with a panel of monoclonal antibodies raised against various β-amyloid fragments. Some of these monoclonal antibodies prevented the formation of β-amyloid and this effect may be related to the localization of the antibody binding sites. Monoclonal antibodies 6C6 and 10D5, and AMY-33, which recognize epitopes spanning the amino acid residues 1-28 of β-amyloid peptide, inhibited its aggregation by 40%-90% when compared with aggregation occurring in the absence of the respective antibodies. The antibodies, 2H3 and 1C2, directed to the regions comprising peptides 1-12 and 13-28, respectively, had a considerably low effect. Identifying the "aggregating epitopes" as sequences that are related to the sites where protein aggregation is initiated and preparing monoclonal antibodies against these regions may facilitate the understanding and prevention of protein aggregation processes. This study may provide a factual basis for using monoclonal antibodies to prevent the β-amyloid formation that is associated with Alzheimer's disease.
KW - Aggregation
KW - Monoclonal antibodies
KW - Protection
KW - β-amyloid
UR - http://www.scopus.com/inward/record.url?scp=0000650806&partnerID=8YFLogxK
U2 - 10.3109/13506129609014365
DO - 10.3109/13506129609014365
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AN - SCOPUS:0000650806
SN - 1350-6129
VL - 3
SP - 130
EP - 133
JO - Amyloid
JF - Amyloid
IS - 2
ER -