Inhibition of respiration and destruction of cytochrome a3 by light in mitochondria and cytochrome oxidase from beef heart

Helga Ninnemann*, W. L. Butler, B. L. Epel

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

38 Scopus citations

Abstract

Irradiation of beef-heart mitochondria and of cytochrome oxidase purified from beef-heart mitochondria with blue light inhibited electron transport from substrate (succinate for the mitochondria and reduced cytochrome c for the cytochrome oxidase) to O2. The irradiation treatment also destroyed cytochrome a3 as assayed by the absorption band for the reduced cyanide-cytochrome a3 complex at 587 nm in the low-temperature absorption spectrum. Irradiation under anaerobic conditions was not inhibitory. Cytochrome a3 was protected against photodestruction if cyanide was present during the irradiation.

Original languageEnglish
Pages (from-to)507-512
Number of pages6
JournalBiochimica et Biophysica Acta - Bioenergetics
Volume205
Issue number3
DOIs
StatePublished - 30 Jun 1970
Externally publishedYes

Funding

FundersFunder number
National Science FoundationGB-6557X
National Institutes of HealthGM-33
American Cancer Society
U.S. Public Health Service

    Fingerprint

    Dive into the research topics of 'Inhibition of respiration and destruction of cytochrome a3 by light in mitochondria and cytochrome oxidase from beef heart'. Together they form a unique fingerprint.

    Cite this