Inhibition of mitogen-induced changes in intracellular fluorescein fluorescence polarization of human peripheral blood lymphocytes by colchicine, vinblastine and cytochalasin B

Avi Eisenthal, Oleg Marder, Beatriz Lifschitz-Mercer, Yehuda Skornick, Reuven Tirosh, Arye Weinreb, Motti Deutsch

Research output: Contribution to journalArticlepeer-review

Abstract

We have previously reported that the exposure of human peripheral blood lymphocytes (PBL) to a variety of stimulants caused rapid changes in intracellular fluorescein fluorescence polarization (IFFP) in the activated cells. In the present study we further analyzed possible mechanisms responsible for the changes in IFFP in PBL exposed to phytohaemagglutinin (PHA) and anti-CD3 antibody. By employing several agents which are known to affect the polymerization of the cytoskeleton we showed that both cytochalasin B, which regulates the microfilaments structure, and vinblastine and colchicine, which affect the microtubules, completely abolished the changes induced in IFFP of human PBL by both PHA and anti-CD3. This effect was dose dependent and was noted at concentrations ranging from 10 to 100 μM of cytochalasin B and 10 μM of vinblastine and colchicine. The effect of these cytoskeleton modulators occurred within 20 minutes after the initiation of activation with PHA. Our results indicate that activation with PHA and anti-CD3 causes early changes in the microtubules and microfilaments components of the cytoskeleton. The possible application of IFFP measurement in analyzing early changes in the cytoskeleton following cell activation is discussed.

Original languageEnglish
Pages (from-to)159-166
Number of pages8
JournalCell Structure and Function
Volume21
Issue number3
DOIs
StatePublished - Jun 1996

Keywords

  • Activation
  • Cytoskeleton
  • Fluorescence
  • Human
  • Lymphocytes

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