TY - JOUR
T1 - Inhibition of CT-26 Murine Adenocarcinoma Growth in the Rectum of Mice Treated with Recombinant Human Interleukin-6
AU - Rabau, Micha
AU - Kashtan, Hanoch
AU - Baron, Shoshana
AU - Yossiphov, Josef
AU - Skornik, Yehuda
AU - Revel, Michel
AU - Eisenthal, Avi
PY - 1994/5
Y1 - 1994/5
N2 - In the present study we evaluated the antitumor effects of recombinant human interleukin-6 (rhIL-6), expressed in Chinese hamster ovary cells, in a murine primary tumor model. We showed that treatment with rhIL-6 substantially inhibited the implantation and growth rates of CT-26 adenocar-cinoma tumor cells in the rectal submucosa of syngeneic mice. This effect was achieved by injecting rhIL-6 for 7 consecutive days starting 1 day prior to tumor inoculation. No obvious antitumor effect was noted when rhIL-6 injections started 5 days after tumor inoculation. Analysis of the mechanisms by which rhIL-6 exerts its antitumor effects did not reveal a direct antitumor effect on CT-26 tumor cells or the up-regulation of major histocompatibility complex antigens on these cells. However, infiltration of lymphocytes at the tumor site was observed. Increase of carcinoembryonic antigen by IL-6 was clearly seen in human HT-29 colon carcinoma cells. The possible application of these results for adjuvant immunotherapy of selected colorectal patients and prevention of reimplantation of tumor cells disseminated during surgery is discussed.
AB - In the present study we evaluated the antitumor effects of recombinant human interleukin-6 (rhIL-6), expressed in Chinese hamster ovary cells, in a murine primary tumor model. We showed that treatment with rhIL-6 substantially inhibited the implantation and growth rates of CT-26 adenocar-cinoma tumor cells in the rectal submucosa of syngeneic mice. This effect was achieved by injecting rhIL-6 for 7 consecutive days starting 1 day prior to tumor inoculation. No obvious antitumor effect was noted when rhIL-6 injections started 5 days after tumor inoculation. Analysis of the mechanisms by which rhIL-6 exerts its antitumor effects did not reveal a direct antitumor effect on CT-26 tumor cells or the up-regulation of major histocompatibility complex antigens on these cells. However, infiltration of lymphocytes at the tumor site was observed. Increase of carcinoembryonic antigen by IL-6 was clearly seen in human HT-29 colon carcinoma cells. The possible application of these results for adjuvant immunotherapy of selected colorectal patients and prevention of reimplantation of tumor cells disseminated during surgery is discussed.
KW - Carcinoembryonic antigen.
KW - Colon carcinoma cells
KW - Interleukin-6
KW - Tumor growth and implantation
UR - http://www.scopus.com/inward/record.url?scp=0028243588&partnerID=8YFLogxK
U2 - 10.1097/00002371-199405000-00004
DO - 10.1097/00002371-199405000-00004
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AN - SCOPUS:0028243588
SN - 1524-9557
VL - 15
SP - 257
EP - 264
JO - Journal of Immunotherapy
JF - Journal of Immunotherapy
IS - 4
ER -