@article{a11d5bd2ae484ede9a8aef6b7e48ec9f,
title = "Inhibition of anxiety in rats by antisense to cholecystokinin precursor protein",
abstract = "Background: Cholecystokinin (CCK) and its analogs generates anxiety in humans and measurable anxiety-like behaviors in rats. CCK receptor blockers have had mixed results as a treatment approach for anxiety disorders. Since CCK is a peptide, we explored another strategy to reduce CCK levels in brain by antisense oligodeoxynucleotide inhibition of DNA transcription or messenger RNA (mRNA) translation for CCK precursor protein. Methods: Antisense oligodeoxynucleotide complementary to the start coding region of rat CCK-precursor was intracerebroventricularly (icv) infused into rats three times at 24-hour intervals. Control groups received infusions of either a scramble sequence oligodeoxynucleotide or vehicle. On the fourth day, rats were assessed in the elevated plus maze paradigm. Results: Compared to vehicle and scramble sequence oligodeoxynucleotide control, icv CCK-antisense exogenous administration for 3 days significantly diminished anxiety behavior in rats. Conclusions: Antisense inhibition of CCK-mediated anxiety could have therapeutic potential in human anxiety disorders.",
keywords = "Animal model, Antisense oligodeoxynucleotide, Anxiety, Cholecystokinin",
author = "Hagit Cohen and Zeev Kaplan and Moshe Kotler",
note = "Funding Information: The work was supported by the National Institute for Psychobiology in Israel Grant #16-96. ",
year = "1998",
month = nov,
day = "1",
doi = "10.1016/S0006-3223(98)00010-9",
language = "אנגלית",
volume = "44",
pages = "915--917",
journal = "Biological Psychiatry",
issn = "0006-3223",
publisher = "Elsevier Inc.",
number = "9",
}