TY - JOUR
T1 - Inhibition of antibody-dependent stimulation of lipoteichoic acid-treated human monocytes and macrophages by polyglycerolphosphate-reactive peptides
AU - Gargir, A.
AU - Ofek, I.
AU - Hasty, D.
AU - Meron-Sudai, S.
AU - Tsubery, H.
AU - Keisari, Y.
AU - Nissim, A.
PY - 2001
Y1 - 2001
N2 - By itself,lipoteichoic acid (LTA) obtained from S. pyogenes, S. aureus, or E. hirae poorly stimulated cytokine production by macrophages, whereas in the presence of anti-polyglycerol phosphate (PGP), the cells secreted significant amounts of IL-6. Two peptides constructed from the deduced sequence of the selected anti-PGP phage-antibody's complementary-determining region 3 of the variable heavy chain (VH-CDR3) reacted specifically with PGP. The monomeric form of the peptides markedly inhibited cytokine production by macrophages pretreated with LTA and anti-LTA. In contrast, the polyvalent form of biotinylated peptides complex with streptavidin-induced cytokine production by the LTA-treated macrophages. The data taken together support the concept that cross-linking of macrophage-bound LTA by anti-PGP is required for cytokine release by these cells. Importantly, these studies identified small, PGP-reactive peptides as potential tools in reducing this proinflammatory process.
AB - By itself,lipoteichoic acid (LTA) obtained from S. pyogenes, S. aureus, or E. hirae poorly stimulated cytokine production by macrophages, whereas in the presence of anti-polyglycerol phosphate (PGP), the cells secreted significant amounts of IL-6. Two peptides constructed from the deduced sequence of the selected anti-PGP phage-antibody's complementary-determining region 3 of the variable heavy chain (VH-CDR3) reacted specifically with PGP. The monomeric form of the peptides markedly inhibited cytokine production by macrophages pretreated with LTA and anti-LTA. In contrast, the polyvalent form of biotinylated peptides complex with streptavidin-induced cytokine production by the LTA-treated macrophages. The data taken together support the concept that cross-linking of macrophage-bound LTA by anti-PGP is required for cytokine release by these cells. Importantly, these studies identified small, PGP-reactive peptides as potential tools in reducing this proinflammatory process.
KW - LTA
KW - Macrophage stimulation
KW - PGP
KW - Phage display
KW - Streptococci
UR - http://www.scopus.com/inward/record.url?scp=0034793199&partnerID=8YFLogxK
U2 - 10.1189/jlb.70.4.537
DO - 10.1189/jlb.70.4.537
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AN - SCOPUS:0034793199
SN - 0741-5400
VL - 70
SP - 537
EP - 542
JO - Journal of Leukocyte Biology
JF - Journal of Leukocyte Biology
IS - 4
ER -