TY - JOUR
T1 - Inhibition by CaNa2EDTA of local tissue damage induced by Bothrops asper (terciopelo) venom
T2 - Application in horse immunization for antivenom production
AU - León, Guillermo
AU - Estrada, Ricardo
AU - Chaves, Fernando
AU - Rojas, Gustavo
AU - Ovadia, Michael
AU - Gutiérrez, José María
N1 - Funding Information:
The authors thank Jorge Sanabria, Javier Núñez, Marjorie Romero and the Stable, Plasma Fractionation and Quality Control staff of Instituto Clodomiro Picado for their collaboration in this work, Dr Bruno Lomonte for critically reading the manuscript and Dr Hui Fan (Instituto Butantan) for discussions on the statistical analysis of clinical observations. This study was supported by AID (grant DHR-5544-6-00-1064-00) and by Vicerrectorı́a de Investigación, Universidad de Costa Rica (project 741-89-057).
PY - 1998/3/26
Y1 - 1998/3/26
N2 - The ability of the chelating agent CaNa2EDTA to inhibit local tissue damage induced by Bothrops asper venom was studied in mice and in horses used for polyvalent (Crotalinae) antivenom production. CaNa2EDTA was devoid of toxicity when injected i.m. or s.c. inducing only a mild edema. Preincubation of B. asper venom with CaNa2EDTA inhibited hemorrhagic and dermonecrotic activities, but did not reduce edema-forming and myotoxic effects. A group of horses initially immunized with native venoms developed less severe local tissue reactions when injected with booster doses of venom and CaNa2EDTA than when receiving booster injections of venom alone, although they showed a similar antivenom response. Moreover, antivenoms produced from plasmas of horses that received booster injections of either venom alone or venom plus CaNa2EDTA had similar neutralizing activity against lethal, hemorrhagic and coagulant effects induced by B. asper venom. The similar antibody response was corroborated by Western blotting using crude venom and by an ELISA that estimates anti-myotoxin titer. It is concluded that the chelating agent CaNa2EDTA reduces the extent of local tissue damage induced by B. asper venom, without affecting the immune response of horses against pharmacologically-relevant venom components.
AB - The ability of the chelating agent CaNa2EDTA to inhibit local tissue damage induced by Bothrops asper venom was studied in mice and in horses used for polyvalent (Crotalinae) antivenom production. CaNa2EDTA was devoid of toxicity when injected i.m. or s.c. inducing only a mild edema. Preincubation of B. asper venom with CaNa2EDTA inhibited hemorrhagic and dermonecrotic activities, but did not reduce edema-forming and myotoxic effects. A group of horses initially immunized with native venoms developed less severe local tissue reactions when injected with booster doses of venom and CaNa2EDTA than when receiving booster injections of venom alone, although they showed a similar antivenom response. Moreover, antivenoms produced from plasmas of horses that received booster injections of either venom alone or venom plus CaNa2EDTA had similar neutralizing activity against lethal, hemorrhagic and coagulant effects induced by B. asper venom. The similar antibody response was corroborated by Western blotting using crude venom and by an ELISA that estimates anti-myotoxin titer. It is concluded that the chelating agent CaNa2EDTA reduces the extent of local tissue damage induced by B. asper venom, without affecting the immune response of horses against pharmacologically-relevant venom components.
UR - http://www.scopus.com/inward/record.url?scp=0031596131&partnerID=8YFLogxK
U2 - 10.1016/S0041-0101(97)00114-1
DO - 10.1016/S0041-0101(97)00114-1
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AN - SCOPUS:0031596131
SN - 0041-0101
VL - 36
SP - 321
EP - 331
JO - Toxicon
JF - Toxicon
IS - 2
ER -