TY - JOUR
T1 - Influence of mechanical tail-detachment techniques of schistosome cercariae on the production, viability, and infectivity of resultant schistosomula
T2 - A comparative study
AU - Gold, D.
AU - Flescher, E.
PY - 2000
Y1 - 2000
N2 - Two decaudation methods used by Schistosoma mansoni cercariae for the production of schistosomula by in vitro transformation were comparatively tested. Both low-pressure syringe passage and vortex mixing of the cercariae produced schistosomula of similar percentages of viability, and the levels of worm recovery following subcutaneous injection in mice were also comparable, though low. By all criteria tested, including simplicity and time requirement, the two methods are equally efficient and are well applicable for mass production of schistosomula.
AB - Two decaudation methods used by Schistosoma mansoni cercariae for the production of schistosomula by in vitro transformation were comparatively tested. Both low-pressure syringe passage and vortex mixing of the cercariae produced schistosomula of similar percentages of viability, and the levels of worm recovery following subcutaneous injection in mice were also comparable, though low. By all criteria tested, including simplicity and time requirement, the two methods are equally efficient and are well applicable for mass production of schistosomula.
UR - http://www.scopus.com/inward/record.url?scp=0033826557&partnerID=8YFLogxK
U2 - 10.1007/s004360000199
DO - 10.1007/s004360000199
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AN - SCOPUS:0033826557
SN - 0932-0113
VL - 86
SP - 570
EP - 572
JO - Parasitology Research
JF - Parasitology Research
IS - 7
ER -
