Influence of aptamer-enclosed silver nanocluster on the prevention of biofilm by bacillus thuringiensis

Bidisha Sengupta*, Sudarson Sekhar Sinha, Bianca L. Garner, Istvan Arany, Christa Corley, Keith Cobb, Elrica Brown, Paresh Chandra Ray

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


The aim of the present study was to determine the effectiveness of silver nanocluster (Ag-NC) against biofilm of Bacillus thuringiensis (BT). A 60-bases long DNA aptamer specific for BT, tied to the 5′ end of a 16-bases long cytosine-rich DNA oligonucleotide, was chosen as template for growing the Ag-NC. Absorption and fluorescence spectroscopic studies confirmed the formation of the fluorescent Ag-NC on the DNA template. A comparative study was then performed on a series of concentrations of the applied agents, like Ag+ ions and DNA-enclosed Ag-NC in absence and presence of methyl-beta-cyclodextrin, on the prevention of biofilm by BT. Quantification of the biofilm formation in 96-well microtiter plates was performed by means of crystal violet staining, which along with phase contrast and transmission electron microscopy indicated that as little as 5 μM of cyclodextrin-conjugated DNA-Ag-NC was sufficient to inhibit the biofilm formation. Implications of these results are discussed. In conclusion, cyclodextrin-bound aptamer enclosed Ag-NC enabled biofilm control, which opens the door to a new theme and paradigm of biofilm prevention.

Original languageEnglish
Pages (from-to)1054-1060
Number of pages7
JournalNanoscience and Nanotechnology Letters
Issue number12
StatePublished - Dec 2016
Externally publishedYes


  • Cyclodextrin
  • Fluorescence
  • Phase contrast imaging
  • TEM


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