TY - JOUR
T1 - Infectious viral DNA of murine leukemia virus
AU - Smotkin, D.
AU - Gianni, A. M.
AU - Rozenblatt, S.
AU - Weinberg, R. A.
PY - 1975
Y1 - 1975
N2 - A fraction of the unintegrated viral DNA that appears early after infection of mouse cells by Moloney leukemia virus is infectious. The infectivity could be demonstrated by an XC plaque assay of the cells exposed to DNA co precipitated with calcium phosphate. The number of plaques deriving from closed circular, supercoiled DNA was proportional to the concentration of added DNA, indicating that a single DNA molecule of about 5.5 x 106 daltons carries all the viral information. Nonsupercoiled viral DNA is also infectious; these molecules appear to be largely double stranded and 5 to 6 x 106 daltons in mass.
AB - A fraction of the unintegrated viral DNA that appears early after infection of mouse cells by Moloney leukemia virus is infectious. The infectivity could be demonstrated by an XC plaque assay of the cells exposed to DNA co precipitated with calcium phosphate. The number of plaques deriving from closed circular, supercoiled DNA was proportional to the concentration of added DNA, indicating that a single DNA molecule of about 5.5 x 106 daltons carries all the viral information. Nonsupercoiled viral DNA is also infectious; these molecules appear to be largely double stranded and 5 to 6 x 106 daltons in mass.
UR - http://www.scopus.com/inward/record.url?scp=0016829984&partnerID=8YFLogxK
U2 - 10.1073/pnas.72.12.4910
DO - 10.1073/pnas.72.12.4910
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AN - SCOPUS:0016829984
SN - 0027-8424
VL - 72
SP - 4910
EP - 4913
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 12
ER -