Infectious viral DNA of murine leukemia virus

D. Smotkin, A. M. Gianni, S. Rozenblatt, R. A. Weinberg

Research output: Contribution to journalArticlepeer-review


A fraction of the unintegrated viral DNA that appears early after infection of mouse cells by Moloney leukemia virus is infectious. The infectivity could be demonstrated by an XC plaque assay of the cells exposed to DNA co precipitated with calcium phosphate. The number of plaques deriving from closed circular, supercoiled DNA was proportional to the concentration of added DNA, indicating that a single DNA molecule of about 5.5 x 106 daltons carries all the viral information. Nonsupercoiled viral DNA is also infectious; these molecules appear to be largely double stranded and 5 to 6 x 106 daltons in mass.

Original languageEnglish
Pages (from-to)4910-4913
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number12
StatePublished - 1975
Externally publishedYes


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