Induction of the chemokine stromal-derived factor-1 following DNA damage improves human stem cell function

Tanya Ponomaryov; Amnon Peled; Isabelle Petit; Russell S. Taichman; Liliana Habler; Judith Sandbank; Fernando Arenzana-Seisdedos; Aude Magerus; Antonio Caruz; Nobutaka Fujii; Arnon Nagler; Meir Lahav; Martin Szyper-Kravitz; Dov Zipori; Tsvee Lapidot

doi:10.1172/JCI10329

Induction of the chemokine stromal-derived factor-1 following DNA damage improves human stem cell function

Tanya Ponomaryov, Amnon Peled, Isabelle Petit, Russell S. Taichman, Liliana Habler, Judith Sandbank, Fernando Arenzana-Seisdedos, Aude Magerus, Antonio Caruz, Nobutaka Fujii, Arnon Nagler, Meir Lahav, Martin Szyper-Kravitz, Dov Zipori, Tsvee Lapidot^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

530 Scopus citations

Abstract

The chemokine stromal-derived factor-1 (SDF-1) controls many aspects of stem cell function. Details of its regulation and sites of production are currently unknown. We report that in the bone marrow, SDF-1 is produced mainly by immature osteoblasts and endothelial cells. Conditioning with DNAdamaging agents (ionizing irradiation, cyclophosphamide, and 5-fluorouracil) caused an increase in SDF-1 expression and in CXCR4-dependent homing and repopulation by human stem cells transplanted into NOD/SCID mice. Our findings suggest that immature osteoblasts and endothelial cells control stem cell homing, retention, and repopulation by secreting SDF-1, which also participates in host defense responses to DNA damage.

Original language	English
Pages (from-to)	1331-1339
Number of pages	9
Journal	Journal of Clinical Investigation
Volume	106
Issue number	11
DOIs	https://doi.org/10.1172/JCI10329
State	Published - 2000
Externally published	Yes

Funding

Funders	Funder number
National Institute of Dental and Craniofacial Research	R29DE011283

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Ponomaryov, T., Peled, A., Petit, I., Taichman, R. S., Habler, L., Sandbank, J., Arenzana-Seisdedos, F., Magerus, A., Caruz, A., Fujii, N., Nagler, A., Lahav, M., Szyper-Kravitz, M., Zipori, D., & Lapidot, T. (2000). Induction of the chemokine stromal-derived factor-1 following DNA damage improves human stem cell function. Journal of Clinical Investigation, 106(11), 1331-1339. https://doi.org/10.1172/JCI10329

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title = "Induction of the chemokine stromal-derived factor-1 following DNA damage improves human stem cell function",

abstract = "The chemokine stromal-derived factor-1 (SDF-1) controls many aspects of stem cell function. Details of its regulation and sites of production are currently unknown. We report that in the bone marrow, SDF-1 is produced mainly by immature osteoblasts and endothelial cells. Conditioning with DNAdamaging agents (ionizing irradiation, cyclophosphamide, and 5-fluorouracil) caused an increase in SDF-1 expression and in CXCR4-dependent homing and repopulation by human stem cells transplanted into NOD/SCID mice. Our findings suggest that immature osteoblasts and endothelial cells control stem cell homing, retention, and repopulation by secreting SDF-1, which also participates in host defense responses to DNA damage.",

author = "Tanya Ponomaryov and Amnon Peled and Isabelle Petit and Taichman, {Russell S.} and Liliana Habler and Judith Sandbank and Fernando Arenzana-Seisdedos and Aude Magerus and Antonio Caruz and Nobutaka Fujii and Arnon Nagler and Meir Lahav and Martin Szyper-Kravitz and Dov Zipori and Tsvee Lapidot",

year = "2000",

doi = "10.1172/JCI10329",

language = "אנגלית",

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Ponomaryov, T, Peled, A, Petit, I, Taichman, RS, Habler, L, Sandbank, J, Arenzana-Seisdedos, F, Magerus, A, Caruz, A, Fujii, N, Nagler, A , Lahav, M, Szyper-Kravitz, M, Zipori, D & Lapidot, T 2000, 'Induction of the chemokine stromal-derived factor-1 following DNA damage improves human stem cell function', Journal of Clinical Investigation, vol. 106, no. 11, pp. 1331-1339. https://doi.org/10.1172/JCI10329

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AU - Ponomaryov, Tanya

AU - Peled, Amnon

AU - Petit, Isabelle

AU - Taichman, Russell S.

AU - Habler, Liliana

AU - Sandbank, Judith

AU - Arenzana-Seisdedos, Fernando

AU - Magerus, Aude

AU - Caruz, Antonio

AU - Fujii, Nobutaka

AU - Nagler, Arnon

AU - Lahav, Meir

AU - Szyper-Kravitz, Martin

AU - Zipori, Dov

AU - Lapidot, Tsvee

PY - 2000

Y1 - 2000

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AB - The chemokine stromal-derived factor-1 (SDF-1) controls many aspects of stem cell function. Details of its regulation and sites of production are currently unknown. We report that in the bone marrow, SDF-1 is produced mainly by immature osteoblasts and endothelial cells. Conditioning with DNAdamaging agents (ionizing irradiation, cyclophosphamide, and 5-fluorouracil) caused an increase in SDF-1 expression and in CXCR4-dependent homing and repopulation by human stem cells transplanted into NOD/SCID mice. Our findings suggest that immature osteoblasts and endothelial cells control stem cell homing, retention, and repopulation by secreting SDF-1, which also participates in host defense responses to DNA damage.

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Induction of the chemokine stromal-derived factor-1 following DNA damage improves human stem cell function

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