TY - JOUR
T1 - Induction of experimental systemic lupus erythematosus in mice by immunization with a monoclonal anti-la autoantibody
AU - Fricke, Harald
AU - Offen, Daniel
AU - Mendlovic, Shlomo
AU - Shoenfeld, Yehuda
AU - Bakimer, Ronit
AU - Sperling, Joseph
AU - Mozes, Edna
N1 - Funding Information:
H.F. is a recipient of a postdoctoral fellowship from The Minerva Foundation This work was supported by a grant from the United States - Israel Binational Science Foundation (BSF), Jerusalem, Israel, by a grant from the German - Israel Foundation for Scientific Research and Development (G.I F.) and by Yeda Fund
PY - 1990/3
Y1 - 1990/3
N2 - Experimental systemic lupus erythematosus (SLE) in mice can be induced by Immunization either with a human monoclonal antl-DNA antibody bearing the 16/6 idiotype (16/6 Id) or with a mouse monoclonal anti-idlotypic antibody specific for the 16/6 Id. In the present report we Investigated the pathogenic role of a monoclonal anti-La autoantibody In the induction and mediation of experimental SLE in mice. The monoclonal anti-La antibody was derived from a mouse in which experimental SLE was Induced by immunization with the monoclonal anti-16/6 Id antibody. Following Immunization with the anti-La antibody the mice produced antibodies to double-stranded DNA, single-stranded DNA, Sm, SS-A/Ro, SS-B/La, and ribonucleoprotein. Furthermore, even though the anti-La antibody does not express nor react with the 16/6 Id, the Immunized mice produced high titers of anti-16/6 Id antibodies as well as 16/6 Id bearing antibodies. Four months following immunization the mice exhibited significant proteinurla, and kidney sections revealed immune complex deposits on the basement membrane of the glomeruli. These results suggest that anti-La autoantibodies are Involved in the induction and mediation of SLE in mice.
AB - Experimental systemic lupus erythematosus (SLE) in mice can be induced by Immunization either with a human monoclonal antl-DNA antibody bearing the 16/6 idiotype (16/6 Id) or with a mouse monoclonal anti-idlotypic antibody specific for the 16/6 Id. In the present report we Investigated the pathogenic role of a monoclonal anti-La autoantibody In the induction and mediation of experimental SLE in mice. The monoclonal anti-La antibody was derived from a mouse in which experimental SLE was Induced by immunization with the monoclonal anti-16/6 Id antibody. Following Immunization with the anti-La antibody the mice produced antibodies to double-stranded DNA, single-stranded DNA, Sm, SS-A/Ro, SS-B/La, and ribonucleoprotein. Furthermore, even though the anti-La antibody does not express nor react with the 16/6 Id, the Immunized mice produced high titers of anti-16/6 Id antibodies as well as 16/6 Id bearing antibodies. Four months following immunization the mice exhibited significant proteinurla, and kidney sections revealed immune complex deposits on the basement membrane of the glomeruli. These results suggest that anti-La autoantibodies are Involved in the induction and mediation of SLE in mice.
KW - Antinuclear autoantibodies
KW - Experimental autoimmune disease
KW - Idiotypic network
KW - Pathogenic idiotype
UR - http://www.scopus.com/inward/record.url?scp=0025342346&partnerID=8YFLogxK
U2 - 10.1093/intimm/2.3.225
DO - 10.1093/intimm/2.3.225
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AN - SCOPUS:0025342346
SN - 0953-8178
VL - 2
SP - 225
EP - 230
JO - International Immunology
JF - International Immunology
IS - 3
ER -