TY - JOUR
T1 - Induction of exocytosis in permeabilized pituitary cells by α- and β-type protein kinase C
AU - Naor, Z.
AU - Dan-Cohen, H.
AU - Hermon, J.
AU - Limor, R.
PY - 1989
Y1 - 1989
N2 - Protein kinase C is now recognized to comprise a family of closely related subspecies (PKCs). When cultured rat pituitary cells were permeabilized by digitonin for 5 min in the absence of Ca2+, endogenous PKC activity was decreased by 72%. PKC depletion was also achieved by prior treatment (24 hr) with high concentrations of phorbol 12-myristate 13-acetate (PMA). When purified activated brain PKCs were added for 30 min to PMA-pretreated, digitonin-permeabilized cells, only α- and β- but not γ-type PKC stimulated luteinizing hormone release. Since PKC was implicated as a mediator of gonadotropin secretion, gonadotropin-releasing hormone might utilize α and β-type PKCs for stimulation of gonadotropin secretion; α- and β-type PKCs might participate also in other exocytotic responses in diverse biological systems in which PKC was implicated.
AB - Protein kinase C is now recognized to comprise a family of closely related subspecies (PKCs). When cultured rat pituitary cells were permeabilized by digitonin for 5 min in the absence of Ca2+, endogenous PKC activity was decreased by 72%. PKC depletion was also achieved by prior treatment (24 hr) with high concentrations of phorbol 12-myristate 13-acetate (PMA). When purified activated brain PKCs were added for 30 min to PMA-pretreated, digitonin-permeabilized cells, only α- and β- but not γ-type PKC stimulated luteinizing hormone release. Since PKC was implicated as a mediator of gonadotropin secretion, gonadotropin-releasing hormone might utilize α and β-type PKCs for stimulation of gonadotropin secretion; α- and β-type PKCs might participate also in other exocytotic responses in diverse biological systems in which PKC was implicated.
UR - http://www.scopus.com/inward/record.url?scp=0344245863&partnerID=8YFLogxK
U2 - 10.1073/pnas.86.12.4501
DO - 10.1073/pnas.86.12.4501
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
C2 - 2660139
AN - SCOPUS:0344245863
SN - 0027-8424
VL - 86
SP - 4501
EP - 4504
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 12
ER -