Induction of exocytosis in permeabilized pituitary cells by α- and β-type protein kinase C

Z. Naor, H. Dan-Cohen, J. Hermon, R. Limor

Research output: Contribution to journalArticlepeer-review

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Abstract

Protein kinase C is now recognized to comprise a family of closely related subspecies (PKCs). When cultured rat pituitary cells were permeabilized by digitonin for 5 min in the absence of Ca2+, endogenous PKC activity was decreased by 72%. PKC depletion was also achieved by prior treatment (24 hr) with high concentrations of phorbol 12-myristate 13-acetate (PMA). When purified activated brain PKCs were added for 30 min to PMA-pretreated, digitonin-permeabilized cells, only α- and β- but not γ-type PKC stimulated luteinizing hormone release. Since PKC was implicated as a mediator of gonadotropin secretion, gonadotropin-releasing hormone might utilize α and β-type PKCs for stimulation of gonadotropin secretion; α- and β-type PKCs might participate also in other exocytotic responses in diverse biological systems in which PKC was implicated.

Original languageEnglish
Pages (from-to)4501-4504
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume86
Issue number12
DOIs
StatePublished - 1989

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