Induction of autologous mesenchymal stem cells at the bone marrow by low level laser therapy has beneficial effects on the ischemic heart and kidney

H. Tuby, L. Maltz, U. Oron*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Multiple clinical trials were performed recently on the use of various stem cells to the ischemic heart. The general outcome of these trials was that the procedures are safe but improvement in the functional performance of the heart was marginal. Acute renal failure has a 50-80% mortality rate. Currently, treatment options for this life-threatening disease are limited. The aim of the present study was to demonstrate that low level laser therapy (LLLT) application to stem cells at the bone marrow (BM) may have beneficial effects on the infarcted rat heart post myocardial infarction, as well as on the ischemic kidney. LLLT applied to the infarcted area in the heart caused a significant reduction of 39% in the infarct size compared to control infracted, non-laser treated rats. LLLT applied directly to stem cells in the BM caused significant (p<0.001) reduction of 79% in the infarct size compared to control. Ventricular dilatation measurements also showed a marked reduction (74%, p<0.001) in the laser treated rats compared to control. In the group of rats in which LLLT was applied to the BM a significant (p=0.05) elevation of 27-fold in the density of c-kit immunopositive cells (a marker of MSCs) in the infarcted area as compared to control was noticed. Electron microscopy indicated newly formed cardiomyocytes at the hearts of the laser treated rats. Quantitative histomorphometric analysis of the histological sections revealed that dilatation of the renal tubules had been reduced, structural integrity of the renal tubules restored, and there was reduced necrosis in the laser-treated rats as compared to the control non-laser-irradiated group. In conclusion, the present study demonstrates a novel approach of applying LLLT to autologous BM of rats with ischemic heart or kidney in order to induce stem cells that are consequently recruited to the ischemic organs, leading to a marked beneficial effect to the heart post-myocardial infarction. The possibility that this approach can also be applied to other ischemic/injured organs, or organs undergoing degenerative processes, with consequent beneficial effects there too, cannot be ruled out.

Original languageEnglish
Title of host publicationProceedings of the 9th World Association for Laser Therapy Congress, WALT 2012
PublisherMEDIMOND s.r.l.
Pages85-88
Number of pages4
ISBN (Print)9788875876777
StatePublished - 2012
Event9th World Association for Laser Therapy Congress, WALT 2012 - Surfers Paradise, QT Gold Coast, Australia
Duration: 28 Sep 201230 Sep 2012

Publication series

NameProceedings of the 9th World Association for Laser Therapy Congress, WALT 2012

Conference

Conference9th World Association for Laser Therapy Congress, WALT 2012
Country/TerritoryAustralia
CitySurfers Paradise, QT Gold Coast
Period28/09/1230/09/12

Keywords

  • Heart
  • Ischemia
  • Kidney
  • Low level lasers
  • Rat

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