TY - GEN
T1 - Induction of autologous mesenchymal stem cells at the bone marrow by low level laser therapy has beneficial effects on the ischemic heart and kidney
AU - Tuby, H.
AU - Maltz, L.
AU - Oron, U.
PY - 2012
Y1 - 2012
N2 - Multiple clinical trials were performed recently on the use of various stem cells to the ischemic heart. The general outcome of these trials was that the procedures are safe but improvement in the functional performance of the heart was marginal. Acute renal failure has a 50-80% mortality rate. Currently, treatment options for this life-threatening disease are limited. The aim of the present study was to demonstrate that low level laser therapy (LLLT) application to stem cells at the bone marrow (BM) may have beneficial effects on the infarcted rat heart post myocardial infarction, as well as on the ischemic kidney. LLLT applied to the infarcted area in the heart caused a significant reduction of 39% in the infarct size compared to control infracted, non-laser treated rats. LLLT applied directly to stem cells in the BM caused significant (p<0.001) reduction of 79% in the infarct size compared to control. Ventricular dilatation measurements also showed a marked reduction (74%, p<0.001) in the laser treated rats compared to control. In the group of rats in which LLLT was applied to the BM a significant (p=0.05) elevation of 27-fold in the density of c-kit immunopositive cells (a marker of MSCs) in the infarcted area as compared to control was noticed. Electron microscopy indicated newly formed cardiomyocytes at the hearts of the laser treated rats. Quantitative histomorphometric analysis of the histological sections revealed that dilatation of the renal tubules had been reduced, structural integrity of the renal tubules restored, and there was reduced necrosis in the laser-treated rats as compared to the control non-laser-irradiated group. In conclusion, the present study demonstrates a novel approach of applying LLLT to autologous BM of rats with ischemic heart or kidney in order to induce stem cells that are consequently recruited to the ischemic organs, leading to a marked beneficial effect to the heart post-myocardial infarction. The possibility that this approach can also be applied to other ischemic/injured organs, or organs undergoing degenerative processes, with consequent beneficial effects there too, cannot be ruled out.
AB - Multiple clinical trials were performed recently on the use of various stem cells to the ischemic heart. The general outcome of these trials was that the procedures are safe but improvement in the functional performance of the heart was marginal. Acute renal failure has a 50-80% mortality rate. Currently, treatment options for this life-threatening disease are limited. The aim of the present study was to demonstrate that low level laser therapy (LLLT) application to stem cells at the bone marrow (BM) may have beneficial effects on the infarcted rat heart post myocardial infarction, as well as on the ischemic kidney. LLLT applied to the infarcted area in the heart caused a significant reduction of 39% in the infarct size compared to control infracted, non-laser treated rats. LLLT applied directly to stem cells in the BM caused significant (p<0.001) reduction of 79% in the infarct size compared to control. Ventricular dilatation measurements also showed a marked reduction (74%, p<0.001) in the laser treated rats compared to control. In the group of rats in which LLLT was applied to the BM a significant (p=0.05) elevation of 27-fold in the density of c-kit immunopositive cells (a marker of MSCs) in the infarcted area as compared to control was noticed. Electron microscopy indicated newly formed cardiomyocytes at the hearts of the laser treated rats. Quantitative histomorphometric analysis of the histological sections revealed that dilatation of the renal tubules had been reduced, structural integrity of the renal tubules restored, and there was reduced necrosis in the laser-treated rats as compared to the control non-laser-irradiated group. In conclusion, the present study demonstrates a novel approach of applying LLLT to autologous BM of rats with ischemic heart or kidney in order to induce stem cells that are consequently recruited to the ischemic organs, leading to a marked beneficial effect to the heart post-myocardial infarction. The possibility that this approach can also be applied to other ischemic/injured organs, or organs undergoing degenerative processes, with consequent beneficial effects there too, cannot be ruled out.
KW - Heart
KW - Ischemia
KW - Kidney
KW - Low level lasers
KW - Rat
UR - http://www.scopus.com/inward/record.url?scp=84902455464&partnerID=8YFLogxK
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AN - SCOPUS:84902455464
SN - 9788875876777
T3 - Proceedings of the 9th World Association for Laser Therapy Congress, WALT 2012
SP - 85
EP - 88
BT - Proceedings of the 9th World Association for Laser Therapy Congress, WALT 2012
PB - MEDIMOND s.r.l.
T2 - 9th World Association for Laser Therapy Congress, WALT 2012
Y2 - 28 September 2012 through 30 September 2012
ER -
