Individually addressable multi-chamber electroporation platform with dielectrophoresis-assisted cell positioning

Sinwook Park; Gilad Yossifon

doi:10.1109/MEMSYS.2014.6765627

Individually addressable multi-chamber electroporation platform with dielectrophoresis-assisted cell positioning

Sinwook Park, Gilad Yossifon

Technion-Israel Institute of Technology

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

We developed a multifunctional microfluidic platform which integrates electroporation and dielectrophoretic (DEP) trapping/positioning mechanisms. Its feasibility was demonstrated by characterizing and optimizing electroporation efficiency using on-chip spatial gradient of electroporation parameters. For simplicity, human dermal fibroblasts cells with a membrane impermeable fluorescent dye, PI, were used as to indicate electroporation. The results clearly show that the developed microfluidic platform has the potential to achieve high-throughput screening for electroporation with spatial control and uniformity.

Original language	English
Title of host publication	MEMS 2014 - 27th IEEE International Conference on Micro Electro Mechanical Systems
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	268-271
Number of pages	4
ISBN (Print)	9781479935086
DOIs	https://doi.org/10.1109/MEMSYS.2014.6765627
State	Published - 2014
Externally published	Yes
Event	27th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2014 - San Francisco, CA, United States Duration: 26 Jan 2014 → 30 Jan 2014

Publication series

Name	Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)	1084-6999

Conference

Conference	27th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2014
Country/Territory	United States
City	San Francisco, CA
Period	26/01/14 → 30/01/14

Access to Document

10.1109/MEMSYS.2014.6765627

Cite this

Park, S., & Yossifon, G. (2014). Individually addressable multi-chamber electroporation platform with dielectrophoresis-assisted cell positioning. In MEMS 2014 - 27th IEEE International Conference on Micro Electro Mechanical Systems (pp. 268-271). Article 6765628 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MEMSYS.2014.6765627

Park, Sinwook ; Yossifon, Gilad. / Individually addressable multi-chamber electroporation platform with dielectrophoresis-assisted cell positioning. MEMS 2014 - 27th IEEE International Conference on Micro Electro Mechanical Systems. Institute of Electrical and Electronics Engineers Inc., 2014. pp. 268-271 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

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title = "Individually addressable multi-chamber electroporation platform with dielectrophoresis-assisted cell positioning",

abstract = "We developed a multifunctional microfluidic platform which integrates electroporation and dielectrophoretic (DEP) trapping/positioning mechanisms. Its feasibility was demonstrated by characterizing and optimizing electroporation efficiency using on-chip spatial gradient of electroporation parameters. For simplicity, human dermal fibroblasts cells with a membrane impermeable fluorescent dye, PI, were used as to indicate electroporation. The results clearly show that the developed microfluidic platform has the potential to achieve high-throughput screening for electroporation with spatial control and uniformity.",

author = "Sinwook Park and Gilad Yossifon",

year = "2014",

doi = "10.1109/MEMSYS.2014.6765627",

language = "אנגלית",

isbn = "9781479935086",

series = "Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)",

publisher = "Institute of Electrical and Electronics Engineers Inc.",

pages = "268--271",

booktitle = "MEMS 2014 - 27th IEEE International Conference on Micro Electro Mechanical Systems",

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note = "27th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2014 ; Conference date: 26-01-2014 Through 30-01-2014",

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Park, S & Yossifon, G 2014, Individually addressable multi-chamber electroporation platform with dielectrophoresis-assisted cell positioning. in MEMS 2014 - 27th IEEE International Conference on Micro Electro Mechanical Systems., 6765628, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), Institute of Electrical and Electronics Engineers Inc., pp. 268-271, 27th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2014, San Francisco, CA, United States, 26/01/14. https://doi.org/10.1109/MEMSYS.2014.6765627

Individually addressable multi-chamber electroporation platform with dielectrophoresis-assisted cell positioning. / Park, Sinwook ; Yossifon, Gilad.
MEMS 2014 - 27th IEEE International Conference on Micro Electro Mechanical Systems. Institute of Electrical and Electronics Engineers Inc., 2014. p. 268-271 6765628 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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AB - We developed a multifunctional microfluidic platform which integrates electroporation and dielectrophoretic (DEP) trapping/positioning mechanisms. Its feasibility was demonstrated by characterizing and optimizing electroporation efficiency using on-chip spatial gradient of electroporation parameters. For simplicity, human dermal fibroblasts cells with a membrane impermeable fluorescent dye, PI, were used as to indicate electroporation. The results clearly show that the developed microfluidic platform has the potential to achieve high-throughput screening for electroporation with spatial control and uniformity.

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Park S , Yossifon G. Individually addressable multi-chamber electroporation platform with dielectrophoresis-assisted cell positioning. In MEMS 2014 - 27th IEEE International Conference on Micro Electro Mechanical Systems. Institute of Electrical and Electronics Engineers Inc. 2014. p. 268-271. 6765628. (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). doi: 10.1109/MEMSYS.2014.6765627

Individually addressable multi-chamber electroporation platform with dielectrophoresis-assisted cell positioning

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