Individualized immune monitoring of cardiac transplant recipients by noninvasive longitudinal cellular immunity tests

Moshe Israeli*, Tuvia Ben-Gal, Vicktoria Yaari, Andrei Valdman, Israel Matz, Benjamin Medalion, Alexander Battler, Benjamin Sredni, Don Kristt, Tirza Klein

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

Background. Common immunosuppression strategies after heart transplantation (HTx) are based on accepted target drug levels, disregarding that drug levels do not correlate with the individual patient's pharmacokinetics or with the actual immunosuppressive drug effect on the patient. The Immuknow assay is used for immune monitoring and management of organ transplant recipients. This study evaluated the Immuknow assay for longitudinal immune monitoring of HTx patients throughout various clinical settings. Methods. The functional immune response as measured by the Immuknow assay was determined in 327 samples collected from 50 HTx patients at the Rabin Medical Center and was analyzed together with common clinical parameters. Results. The median Immuknow levels measured throughout the infection episodes and the episodes of biopsy-proven acute rejection were 129 and 619 ng ATP/mL, respectively. These values were significantly dissimilar to the median Immuknow level measured during clinical quiescence, which was 351 ng ATP/mL (P<0.05). Calcineurin inhibitors drug-level measurements did not provide a reliable depiction of the patients' immune function, because the median deviation from the recommended drug trough levels range was significantly higher than the median deviation of Immuknow levels from their expected immune response zones. Longitudinal monitoring of Immuknow levels through serial testing proved to be a reliable method for individual patient immune management. Conclusions. The Immuknow assay reliably reflects the cellular immune function of HTx patients, thereby supporting the immune monitoring and management of these patients. Serial longitudinal Immuknow monitoring allows immune management of therapy according to the individual patient's immune status.

Original languageEnglish
Pages (from-to)968-976
Number of pages9
JournalTransplantation
Volume89
Issue number8
DOIs
StatePublished - Apr 2010

Keywords

  • Heart transplantation
  • Immune monitoring
  • Immunosuppression
  • Posttransplant

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