Increasing synthetic serum substitute (SSS) concentrations in P1 glucose/phosphate-free medium improves implantation rate: A comparative study

Dalit Ben-Yosef*, Israel Yovel, Tamar Schwartz, Fuad Azem, Joseph B. Lessing, Ami Amit

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Purpose: To assess the comparative efficacy of IVF medium (MediCult, with 5.2 mM glucose) and a glucose/phosphate-free medium, PI (Irvine Scientific), and to investigate the influence of increasing the serum supplementation (synthetic serum substitute; SSS; Irvine Scientific) to P1 on embryo development and implantation. Methods: Patients were randomly assigned to IVF medium (Group 1, cycles n = 172) or PI supplemented with 10% SSS (Group 2, cycles n = 229) according to the medium scheduled for use on the day of oocyte retrieval. Another 555 IVF consequent cycles (Group 3) were performed using increased SSS concentrations (20%) in P1 medium. Results and Conclusion: In this large series of IVF cycles, we herein demonstrate that significantly higher pregnancy and implantation rates were found when embryos were cultured in glucose/phosphate-free medium P1 supplemented with 20% SSS compared to supplementation with the lower SSS concentration and with IVF medium.

Original languageEnglish
Pages (from-to)588-592
Number of pages5
JournalJournal of Assisted Reproduction and Genetics
Volume18
Issue number11
DOIs
StatePublished - 2001
Externally publishedYes

Keywords

  • Glucose
  • IVF culture medium
  • Implantation rate
  • Pregnancy
  • Serum

Fingerprint

Dive into the research topics of 'Increasing synthetic serum substitute (SSS) concentrations in P1 glucose/phosphate-free medium improves implantation rate: A comparative study'. Together they form a unique fingerprint.

Cite this