TY - JOUR
T1 - Increasing synthetic serum substitute (SSS) concentrations in P1 glucose/phosphate-free medium improves implantation rate
T2 - A comparative study
AU - Ben-Yosef, Dalit
AU - Yovel, Israel
AU - Schwartz, Tamar
AU - Azem, Fuad
AU - Lessing, Joseph B.
AU - Amit, Ami
PY - 2001
Y1 - 2001
N2 - Purpose: To assess the comparative efficacy of IVF medium (MediCult, with 5.2 mM glucose) and a glucose/phosphate-free medium, PI (Irvine Scientific), and to investigate the influence of increasing the serum supplementation (synthetic serum substitute; SSS; Irvine Scientific) to P1 on embryo development and implantation. Methods: Patients were randomly assigned to IVF medium (Group 1, cycles n = 172) or PI supplemented with 10% SSS (Group 2, cycles n = 229) according to the medium scheduled for use on the day of oocyte retrieval. Another 555 IVF consequent cycles (Group 3) were performed using increased SSS concentrations (20%) in P1 medium. Results and Conclusion: In this large series of IVF cycles, we herein demonstrate that significantly higher pregnancy and implantation rates were found when embryos were cultured in glucose/phosphate-free medium P1 supplemented with 20% SSS compared to supplementation with the lower SSS concentration and with IVF medium.
AB - Purpose: To assess the comparative efficacy of IVF medium (MediCult, with 5.2 mM glucose) and a glucose/phosphate-free medium, PI (Irvine Scientific), and to investigate the influence of increasing the serum supplementation (synthetic serum substitute; SSS; Irvine Scientific) to P1 on embryo development and implantation. Methods: Patients were randomly assigned to IVF medium (Group 1, cycles n = 172) or PI supplemented with 10% SSS (Group 2, cycles n = 229) according to the medium scheduled for use on the day of oocyte retrieval. Another 555 IVF consequent cycles (Group 3) were performed using increased SSS concentrations (20%) in P1 medium. Results and Conclusion: In this large series of IVF cycles, we herein demonstrate that significantly higher pregnancy and implantation rates were found when embryos were cultured in glucose/phosphate-free medium P1 supplemented with 20% SSS compared to supplementation with the lower SSS concentration and with IVF medium.
KW - Glucose
KW - IVF culture medium
KW - Implantation rate
KW - Pregnancy
KW - Serum
UR - http://www.scopus.com/inward/record.url?scp=0035682363&partnerID=8YFLogxK
U2 - 10.1023/A:1013156804589
DO - 10.1023/A:1013156804589
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AN - SCOPUS:0035682363
SN - 1058-0468
VL - 18
SP - 588
EP - 592
JO - Journal of Assisted Reproduction and Genetics
JF - Journal of Assisted Reproduction and Genetics
IS - 11
ER -