TY - JOUR
T1 - Increased translatability in a cell-free system of RNA extracted from actinomycin D-treated cultures
AU - Kessler-Icekson, Gania
AU - Yaffe, David
N1 - Funding Information:
assistance of Mrs. Sara Neuman is acknowledged. from the Israel-U. S. Binational Science Foundation, Association, Inc. , USA and the National Institutes
Funding Information:
The excellent technical was supported by grants the Muscular Dystrophy (Grant No. ROl-GM-22767).
PY - 1977/3/7
Y1 - 1977/3/7
N2 - RNA extracted from myogenic cultures treated with actinomycin D was found to be more active in stimulating protein synthesis in the wheat germ cell-free system than RNA from untreated cultures. The rate of incorporation of amino acids was up to 30% higher and the synthesis of actin and of myosin light chains increased by up to 50% when RNA from actinomycin-treated cultures was used. A cell-free system product which affects the rate of translation does not seem to be involved in this phenomenon.
AB - RNA extracted from myogenic cultures treated with actinomycin D was found to be more active in stimulating protein synthesis in the wheat germ cell-free system than RNA from untreated cultures. The rate of incorporation of amino acids was up to 30% higher and the synthesis of actin and of myosin light chains increased by up to 50% when RNA from actinomycin-treated cultures was used. A cell-free system product which affects the rate of translation does not seem to be involved in this phenomenon.
UR - http://www.scopus.com/inward/record.url?scp=0017595013&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(77)91289-X
DO - 10.1016/0006-291X(77)91289-X
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C2 - 849310
AN - SCOPUS:0017595013
SN - 0006-291X
VL - 75
SP - 62
EP - 68
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -