TY - JOUR
T1 - Increase in immune cell infiltration with progression of oral epithelium from hyperkeratosis to dysplasia and carcinoma
AU - Gannot, G.
AU - Gannot, I.
AU - Vered, H.
AU - Buchner, A.
AU - Keisari, Y.
N1 - Funding Information:
This research was supported by the Ministry of Science, Culture and Sport Strategic Research Grant No. 8445. The authors wish to thank Dr Gary L Ellis from the department of Oral & Maxillofacial Pathology at the Armed Forces Institute of Pathology (AFIP) for supplying the parotid gland samples. This study was conducted in partial fulfillment of the requirements for a PhD degree of Gallya Gannot, Sackler Faculty of Medicine, Tel Aviv University, Israel.
PY - 2002
Y1 - 2002
N2 - In the present study, epithelium derived lesions of various pathological manifestations were examined histologically and immunohistochemically for mononuclear cell infiltration. The infiltrate under the transformed epithelium of oral lesions, was examined for differences in the composition of immune mononuclear cells as the epithelium moves from hyperkeratosis through various degrees of dysplasia to squamous cell carcinoma. The study was performed on 53 human tongue tissues diagnosed as hyperkeratosis (11 cases), mild dysptasia (nine cases), moderate and severe dysplasia (14 cases) and squamous cell carcinoma (19 cases). A similar analysis was performed on 30 parotid gland tissues diagnosed as pleomorphic adenoma (14 cases) and carcinoma ex-pleomorphic adenoma (16 cases). Immunohistochemical analysis of various surface markers of the tumour infiltrating immune cells was performed and correlated with the transformation level as defined by morphology and the expression of p53 in the epithelium. The results revealed that, in the tongue lesions, the changes in the epithelium from normal appearance to transformed were accompanied by a corresponding increase in the infiltration of CD4, CD8, CD 14, CD19+20, and HLA/DR positive cells. The most significant change was an increase in B lymphocytes in tongue lesions, that was in accordance with the transformation level (P<0.001). In the salivary gland, a significant number of cases did not show an infiltrate. In cases where an infiltrate was present, a similar pattern was observed and the more malignant tissues exhibited a higher degree of immune cell infiltration.
AB - In the present study, epithelium derived lesions of various pathological manifestations were examined histologically and immunohistochemically for mononuclear cell infiltration. The infiltrate under the transformed epithelium of oral lesions, was examined for differences in the composition of immune mononuclear cells as the epithelium moves from hyperkeratosis through various degrees of dysplasia to squamous cell carcinoma. The study was performed on 53 human tongue tissues diagnosed as hyperkeratosis (11 cases), mild dysptasia (nine cases), moderate and severe dysplasia (14 cases) and squamous cell carcinoma (19 cases). A similar analysis was performed on 30 parotid gland tissues diagnosed as pleomorphic adenoma (14 cases) and carcinoma ex-pleomorphic adenoma (16 cases). Immunohistochemical analysis of various surface markers of the tumour infiltrating immune cells was performed and correlated with the transformation level as defined by morphology and the expression of p53 in the epithelium. The results revealed that, in the tongue lesions, the changes in the epithelium from normal appearance to transformed were accompanied by a corresponding increase in the infiltration of CD4, CD8, CD 14, CD19+20, and HLA/DR positive cells. The most significant change was an increase in B lymphocytes in tongue lesions, that was in accordance with the transformation level (P<0.001). In the salivary gland, a significant number of cases did not show an infiltrate. In cases where an infiltrate was present, a similar pattern was observed and the more malignant tissues exhibited a higher degree of immune cell infiltration.
KW - Carcinoma
KW - Diagnosis
KW - Human
KW - Immune infiltrate
KW - Transformation
UR - http://www.scopus.com/inward/record.url?scp=0037029715&partnerID=8YFLogxK
U2 - 10.1038/sj.bjc.6600282
DO - 10.1038/sj.bjc.6600282
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AN - SCOPUS:0037029715
SN - 0007-0920
VL - 86
SP - 1444
EP - 1448
JO - British Journal of Cancer
JF - British Journal of Cancer
IS - 9
ER -