Incorporation of 125I-iododeoxyuridine into target cells as an assay for cell immunity

Falk Fish, Margalit Yaakubovicz, Isaac P. Witz*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

We assayed cell immunity by measuring the decrease of 125I-iododeoxyuridine (125IUDR) uptake into target cells when incubated with sensitized effector cells. This method was suitable for target cells in monolayer or in suspension cultures. A labeling regimen was used in which 125IUDR was added after a short exposure of the target cells to effector cells. This obviated the need to preplate the target cells in the culture vessel a day before or to prelabel them. Thereby, the test was further simplified and shortened. This method was used successfully in allogeneic and syngeneic (tumor-specific) systems. In the syngeneic systems, lymph node cells (LNC) from mice with a syngeneic 3-methylcholanthrene-induced tumor inhibited 125IUDR incorporation into the corresponding tumor cells. LNC from mice with a different syngeneic 3-methylcholanthrene-induced tumor did not inhibit 125IUDR incorporation into the first tumor cells.

Original languageEnglish
Pages (from-to)1743-1747
Number of pages5
JournalJournal of the National Cancer Institute
Volume53
Issue number6
DOIs
StatePublished - Dec 1974

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