Abstract
The effect of deoxyadenosine (AdR) and 5-fluorodeoxyuridine (FUdR) on the incorporation of 5-bromodeoxyuridine (BUdR) into DNA of thymine-non requiring cells of Escherichia coli was studied. This incorporation renders the cells sensitive to irradiation at near-UV light. It was found that the combination of AdR and FUdR increases incorporation of BUdR and sensitivity to irradiation. An enrichment of auxotrophs by a factor of 105 was obtained from a mixed population grown in minimal medium containing BUdR, AdR and FUdR and irradiated subsequently.
Original language | English |
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Pages (from-to) | 254-262 |
Number of pages | 9 |
Journal | Molecular Genetics and Genomics |
Volume | 106 |
Issue number | 3 |
DOIs | |
State | Published - Sep 1970 |