Incorporation and uptake of thymidine and uridine by hela cells: Effect of DNA extracts prepared from normal human fibroblasts

Rachel Ashkenazi-Ezra, Israel E. Ashkenazi

Research output: Contribution to journalArticlepeer-review

Abstract

The incorporation and uptake of (3H) thymidine into HeLa cells markedly decreased in the presence of nuclear homogenates and DNA extracts that have been derived from normal diploid cell cultures. On the other hand, uridine uptake and incorporation were stimulated under the same conditions. The inhibition could be reversed immediately upon removal of the exogenous fraction from the culture medium. The inhibitory properties of the extracts are propagated by excreted cellular components as well as after DNAase treatment. The inhibitory factor is thermostable, resistant to pronase treatment, and seems to be related to nucleic acid.

Original languageEnglish
Pages (from-to)442-449
Number of pages8
JournalIn Vitro
Volume12
Issue number6
DOIs
StatePublished - Jun 1976

Keywords

  • HeLa cells
  • cell and DNA extracts
  • inhibition of uptake and incorporation
  • thymidine and uridine

Fingerprint

Dive into the research topics of 'Incorporation and uptake of thymidine and uridine by hela cells: Effect of DNA extracts prepared from normal human fibroblasts'. Together they form a unique fingerprint.

Cite this