TY - JOUR
T1 - Incorporation and uptake of thymidine and uridine by hela cells
T2 - Effect of DNA extracts prepared from normal human fibroblasts
AU - Ashkenazi-Ezra, Rachel
AU - Ashkenazi, Israel E.
PY - 1976/6
Y1 - 1976/6
N2 - The incorporation and uptake of (3H) thymidine into HeLa cells markedly decreased in the presence of nuclear homogenates and DNA extracts that have been derived from normal diploid cell cultures. On the other hand, uridine uptake and incorporation were stimulated under the same conditions. The inhibition could be reversed immediately upon removal of the exogenous fraction from the culture medium. The inhibitory properties of the extracts are propagated by excreted cellular components as well as after DNAase treatment. The inhibitory factor is thermostable, resistant to pronase treatment, and seems to be related to nucleic acid.
AB - The incorporation and uptake of (3H) thymidine into HeLa cells markedly decreased in the presence of nuclear homogenates and DNA extracts that have been derived from normal diploid cell cultures. On the other hand, uridine uptake and incorporation were stimulated under the same conditions. The inhibition could be reversed immediately upon removal of the exogenous fraction from the culture medium. The inhibitory properties of the extracts are propagated by excreted cellular components as well as after DNAase treatment. The inhibitory factor is thermostable, resistant to pronase treatment, and seems to be related to nucleic acid.
KW - HeLa cells
KW - cell and DNA extracts
KW - inhibition of uptake and incorporation
KW - thymidine and uridine
UR - http://www.scopus.com/inward/record.url?scp=0017168370&partnerID=8YFLogxK
U2 - 10.1007/BF02806024
DO - 10.1007/BF02806024
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AN - SCOPUS:0017168370
SN - 0073-5655
VL - 12
SP - 442
EP - 449
JO - In Vitro
JF - In Vitro
IS - 6
ER -