In Vivo Tracking of Bacterial Colonization in Different Murine Models Using Bioluminescence: The Example of Salmonella

Michaël Koczerka; Isabelle Lantier; Anne Pinard; Marie Morillon; Justine Deperne; Ohad Gal-Mor; Olivier Grépinet; Isabelle Virlogeux-Payant

doi:10.1007/978-1-0716-1971-1_19

In Vivo Tracking of Bacterial Colonization in Different Murine Models Using Bioluminescence: The Example of Salmonella

Michaël Koczerka, Isabelle Lantier, Anne Pinard, Marie Morillon, Justine Deperne, Ohad Gal-Mor, Olivier Grépinet, Isabelle Virlogeux-Payant^*

^*Corresponding author for this work

Clinical Microbiology and Immunology

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

3 Scopus citations

Abstract

Applications of bioluminescence for the in vivo study of pathogenic microorganisms are numerous, ranging from the quantification of virulence gene expression to measuring the effect of antimicrobial molecules on the colonization of tissues and organs by the pathogen. Most studies are performed in mice, but recent works demonstrate that this technique is applicable to larger animals like fish, guinea pigs, ferrets, and chickens. Here, we describe the construction and the utilization of a constitutively luminescent strain of Salmonella Typhimurium to monitor in vivo and ex vivo the colonization of mice in the gastroenteritis, typhoid fever, and asymptomatic carriage models of Salmonella infection.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	235-248
Number of pages	14
DOIs	https://doi.org/10.1007/978-1-0716-1971-1_19
State	Published - 2022

Publication series

Name	Methods in Molecular Biology
Volume	2427
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Funding

Funders	Funder number
Horizon 2020 Framework Programme	731014
Agence Nationale de la Recherche
Université François-Rabelais

Keywords

Bioluminescence
In vivo imaging
Infection kinetics
Murine models
Salmonella Typhimurium

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10.1007/978-1-0716-1971-1_19

Cite this

Koczerka, M., Lantier, I., Pinard, A., Morillon, M., Deperne, J., Gal-Mor, O., Grépinet, O., & Virlogeux-Payant, I. (2022). In Vivo Tracking of Bacterial Colonization in Different Murine Models Using Bioluminescence: The Example of Salmonella. In Methods in Molecular Biology (pp. 235-248). (Methods in Molecular Biology; Vol. 2427). Humana Press Inc.. https://doi.org/10.1007/978-1-0716-1971-1_19

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Koczerka, M, Lantier, I, Pinard, A, Morillon, M, Deperne, J, Gal-Mor, O, Grépinet, O & Virlogeux-Payant, I 2022, In Vivo Tracking of Bacterial Colonization in Different Murine Models Using Bioluminescence: The Example of Salmonella. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 2427, Humana Press Inc., pp. 235-248. https://doi.org/10.1007/978-1-0716-1971-1_19

In Vivo Tracking of Bacterial Colonization in Different Murine Models Using Bioluminescence: The Example of Salmonella. / Koczerka, Michaël; Lantier, Isabelle; Pinard, Anne et al.
Methods in Molecular Biology. Humana Press Inc., 2022. p. 235-248 (Methods in Molecular Biology; Vol. 2427).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

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T2 - The Example of Salmonella

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AU - Deperne, Justine

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AU - Grépinet, Olivier

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In Vivo Tracking of Bacterial Colonization in Different Murine Models Using Bioluminescence: The Example of Salmonella

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